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CoraLite®594-conjugated FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11531

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863



Tested Applications

Positive IF detected inA549 cells, HUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 11531-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-11531 targets FBF1 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBF1 fusion protein Ag2106
Full Name Fas (TNFRSF6) binding factor 1
Calculated molecular weight 830 aa, 90 kDa
Observed molecular weight 130 kDa, 90 kDa, 70 kDa
GenBank accession numberBC023549
Gene symbol FBF1
Gene ID (NCBI) 85302
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Protocols

Product Specific Protocols
IF protocol for CL594 FBF1 antibody CL594-11531Download protocol
Standard Protocols
Click here to view our Standard Protocols