FBL Polyclonal antibody

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  WB analysis using 16021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16021-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16021-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 16021-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 16021-1-AP

  IP result of anti-FBL (IP:16021-1-AP, 4ug; Detection:16021-1-AP 1:500) with A375 cells lysate 3600ug.

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  IHC staining of mouse brain using 16021-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 16021-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FBL (16021-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

FBL Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16021-1-AP

Publications(32)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, RIP, ELISA

Synonyms

EC:2.1.1.-, FIB, FIB1, fibrillarin, FLRN

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Tested Applications

• Positive WB detected in: A375 cells, mouse liver tissue, mosue kidney tissue
• Positive IP detected in: A375 cells
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• RIP: See 4 publications below

Product Information

16021-1-AP targets FBL in WB, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBL fusion protein Ag8841
• Full Name: fibrillarin
• Calculated Molecular Weight: 321 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC019260
• Gene Symbol: FBL
• Gene ID (NCBI): 2091
• RRID: AB_2105788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22087
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; Oxidative cyclization reagents reveal tryptophan cation-π interactions; Authors - Xiao Xie
human	WB,RIP,IF	Mol Cell; lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing; Authors - Yu-Meng Sun
human	WB	Mol Cell; LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.; Authors - Xin Wang ; KD Validated
human	WB	Breast Cancer Res; U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression; Authors - Wenrong Zhang
human	WB,IF	Cell Biol Toxicol; SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells; Authors - Jingyi Cui
	IF	Commun Biol; Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus; Authors - Tetsuya Yamamoto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, U2OS cells

FH

WEI (Verified Customer) (03-12-2020)

Good, strong band for WB and correct staining for cardiomyocytes.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse Heart

FH

Lauren (Verified Customer) (02-20-2020)

Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mIMCD3

FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)

FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human satelllite muscle cells

FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)