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FBL Polyclonal antibody
FBL Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 16021-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, mouse kidney tissue, mouse liver tissue |
Positive IP detected in | A375 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 8 publications below |
IF | See 3 publications below |
RIP | See 2 publications below |
Product Information
16021-1-AP targets FBL in WB, RIP, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FBL fusion protein Ag8841 |
Full Name | fibrillarin |
Calculated molecular weight | 321 aa, 34 kDa |
Observed molecular weight | 34 kDa |
GenBank accession number | BC019260 |
Gene symbol | FBL |
Gene ID (NCBI) | 2091 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FBL antibody 16021-1-AP | Download protocol |
IF protocol for FBL antibody 16021-1-AP | Download protocol |
IP protocol for FBL antibody 16021-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.
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Oncotarget Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis. | ||
Front Immunol RNA 2'-O-Methyltransferase Fibrillarin Facilitates Virus Entry Into Macrophages Through Inhibiting Type I Interferon Response.
| ||
J Virol Zika Virus Subverts Stress Granules To Promote and Restrict Viral Gene Expression. | ||
RNA Identification and mapping of post-transcriptional modifications on the HIV-1 antisense transcript Ast in human cells. | ||
J Mol Biol Proteomic characterization of the nucleolar linker histone H1 interaction network. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH WEI (Verified Customer) (03-12-2020) | Good, strong band for WB and correct staining for cardiomyocytes.
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FH Lauren (Verified Customer) (02-20-2020) | Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.
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FH Baptiste (Verified Customer) (09-05-2019) | This antibody works well in western blot
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