• Featured Product
  • KD/KO Validated

FBL Polyclonal antibody

FBL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16021-1-AP

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1



Tested Applications

Positive WB detected inA375 cells, mouse liver tissue, mosue kidney tissue
Positive IP detected inA375 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16021-1-AP targets FBL in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBL fusion protein Ag8841
Full Name fibrillarin
Calculated molecular weight 321 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019260
Gene symbol FBL
Gene ID (NCBI) 2091
RRIDAB_2105788
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FBL antibody 16021-1-APDownload protocol
IHC protocol for FBL antibody 16021-1-APDownload protocol
IF protocol for FBL antibody 16021-1-APDownload protocol
IP protocol for FBL antibody 16021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.

Authors - Xin Wang
  • KD Validated
humanWB,IF

Cell Biol Toxicol

SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells

Authors - Jingyi Cui
IF

Commun Biol

Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus

Authors - Tetsuya Yamamoto
humanWB

Oncotarget

Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis.

Authors - Xing Liu
human,mouseWB

Front Immunol

RNA 2'-O-Methyltransferase Fibrillarin Facilitates Virus Entry Into Macrophages Through Inhibiting Type I Interferon Response.

Authors - Panpan Li
  • KD Validated
humanRIP

Mol Carcinog

SNORD60 promotes the tumorigenesis and progression of endometrial cancer through binding PIK3CA and regulating PI3K/AKT/mTOR signaling pathway

Authors - Wu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tatyana (Verified Customer) (03-11-2023)

Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, U2OS cells
FH

WEI (Verified Customer) (03-12-2020)

Good, strong band for WB and correct staining for cardiomyocytes.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Heart
FH

Lauren (Verified Customer) (02-20-2020)

Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mIMCD3
FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)
FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human satelllite muscle cells
FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)