FBL Polyclonal antibody

• Featured Product
• KD/KO Validated

FBL Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IF, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (3)

Cat no : 16021-1-AP

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1

---

Validation Data Gallery

• 

  WB analysis using 16021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16021-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16021-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 16021-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 16021-1-AP

  IP Result of anti-FBL (IP:16021-1-AP, 4ug; Detection:16021-1-AP 1:500) with A375 cells lysate 3600ug.

• 

  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: A375 cells, mouse kidney tissue, mouse liver tissue
• Positive IP detected in: A375 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• RIP: See 2 publications below

Product Information

16021-1-AP targets FBL in WB, RIP, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBL fusion protein Ag8841
• Full Name: fibrillarin
• Calculated molecular weight: 321 aa, 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC019260
• Gene symbol: FBL
• Gene ID (NCBI): 2091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Mol Cell; LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.; Authors - Xin Wang ; KD Validated
human	WB	Oncotarget; Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis.; Authors - Xing Liu
human,mouse	WB	Front Immunol; RNA 2'-O-Methyltransferase Fibrillarin Facilitates Virus Entry Into Macrophages Through Inhibiting Type I Interferon Response.; Authors - Panpan Li ; KD Validated
human	WB	J Virol; Zika Virus Subverts Stress Granules To Promote and Restrict Viral Gene Expression.; Authors - Gaston Bonenfant
human	RIP	RNA; Identification and mapping of post-transcriptional modifications on the HIV-1 antisense transcript Ast in human cells.; Authors - Mariana Estevez
human		J Mol Biol; Proteomic characterization of the nucleolar linker histone H1 interaction network.; Authors - Heather J Szerlong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

WEI (Verified Customer) (03-12-2020)

Good, strong band for WB and correct staining for cardiomyocytes.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse Heart

FH

Lauren (Verified Customer) (02-20-2020)

Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mIMCD3

FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)

FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human satelllite muscle cells

FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)