FER Polyclonal antibody

FER Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 25287-1-AP

Synonyms

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer



Tested Applications

Positive WB detected inNIH/3T3 cells, A431 cells, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25287-1-AP is FER Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FER fusion protein Ag18173
Full Name fer (fps/fes related) tyrosine kinase
Calculated molecular weight 822 aa, 95 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC017060
Gene symbol FER
Gene ID (NCBI) 2241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FER antibody 25287-1-APDownload protocol
IHC protocol for FER antibody 25287-1-APDownload protocol
IF protocol for FER antibody 25287-1-APDownload protocol
IP protocol for FER antibody 25287-1-APDownload protocol
FC protocol for FER antibody 25287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.

Authors - Xue Yang