FGF2 Polyclonal antibody

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  WB analysis using 30333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30333-1-AP (FGF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SKOV-3 using 30333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using FGF-2 antibody (30333-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

FGF2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30333-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FGF-2, fibroblast growth factor 2, FGFB, FGF basic, FGF B

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Tested Applications

• Positive WB detected in: K-562 cells, SKOV-3 cells, U-87 MG cells
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

30333-1-AP targets FGF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28653

  Product name: Recombinant human FGF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 143-275 aa of NM_002006

  Sequence: MPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPG

• Full Name: fibroblast growth factor 2 (basic)
• Calculated Molecular Weight: 31 aa
• Observed Molecular Weight: 21 kDa，23 kDa
• GenBank Accession Number: NM_002006
• Gene Symbol: FGF2
• Gene ID (NCBI): 2247
• RRID: AB_3669711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09038
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Bioengineering (Basel); Shark Cartilage-Derived Anti-Angiogenic Peptide Inhibits Corneal Neovascularization; Authors - Yunxian Li