Tested Applications
| Positive WB detected in | mouse brain tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30202-1-AP targets FGFR3 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | fibroblast growth factor receptor 3 |
| Calculated Molecular Weight | 88kd |
| Observed Molecular Weight | 115 kDa |
| Gene Symbol | FGFR3 |
| Gene ID (NCBI) | 2261 |
| RRID | AB_3742331 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22607 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FGFR3 antibody 30202-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FGFR3 Polyclonal antibody (Cat# 30202-1-AP) has 1 total citation. It appears in Investigative Ophthalmology & Visual Science, a journal focused on ophthalmology and visual science. The cited study investigates stromal-epithelial crosstalk and identifies the FGF21-FGFR3 axis as a key driver of corneal wound healing, placing this citation within the research fields of ophthalmology, corneal biology, and growth factor signaling.
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Systematic Analysis of Stromal-Epithelial Crosstalk Reveals the FGF21-FGFR3 Axis as a Key Driver of Corneal Wound Healing. |
