FIS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FIS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (4)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

133

Reviews (4)

Cat no : 10956-1-AP

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

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Validation Data Gallery

• 

  WB analysis using 10956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 10956-1-AP

  WB result of FIS1 antibody (10956-1-AP, 1:8,000) with si-Control and si-FIS1 transfected HeLa cells.

• 

  IF Staining of Hepa1-6 cells using 10956-1-AP

  IF result of anti-FIS1(10956-1-AP,1:100) with Hepa1-6 cell by Dr. Steven Eugene Smith. Mitochondrion outer membrane (Green) Stain.

• 

  IF Staining of HepG2 using 10956-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10956-1-AP (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IP experiment of HeLa using 10956-1-AP

  IP Result of anti-FIS1 (IP:10956-1-AP, 3ug; Detection:10956-1-AP 1:500) with HeLa cells lysate 3000ug.

• 

  IHC staining of rat brain using 10956-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10956-1-AP (FIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, pig brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue, mouse brain tissue, Jurkat cells, mouse spleen, rat spleen
• Positive IP detected in: HeLa cells
• Positive IHC detected in: rat brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hepa1-6 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 121 publications below
• IHC: See 5 publications below
• IF: See 18 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10956-1-AP targets FIS1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: bovine, Chinese hamster ovary (CHO) cell, hamster, human, mouse, pig, rat, squirrel
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FIS1 fusion protein Ag1409
• Full Name: fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
• Calculated molecular weight: 17 kDa
• Observed molecular weight: 17 kDa
• GenBank accession number: BC009428
• Gene symbol: FIS1
• Gene ID (NCBI): 51024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

Publications

Species	Application	Title
human		Proc Natl Acad Sci U S A; Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.; Authors - Chih-Wei Chen
squirrel	WB	J Cell Physiol; Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.; Authors - Zhe Wang
human	WB,IF	Arch Toxicol; PMID: 30659323; Authors - Qian Zhao
rat	WB	Front Pharmacol; PMID: 30809145; Authors - Lidong Zhao
human	IF	Free Radic Biol Med; Low-dose oral copper treatment changes the hippocampal phosphoproteomic profile and perturbs mitochondrial function in a mouse model of Alzheimer's disease.; Authors - Chongyang Chen
human	WB	Nat Commun; ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.; Authors - Yuanyuan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Megan (Verified Customer) (06-22-2021)

RPE cell over-expressing pcDNA hFis1. Note 1:500 was used here to detect over-expressed Fis1, but we also use 1:100 when detecting endogenous Fis1. Also successfully used to detect endogenous Fis1 levels via Western Blot (used @ 1:1000).

• Applications: Western Blot,Immunofluorescence
• Primary Antibody Dilution: 1:100 - 1:500
• Cell Tissue Type: RPE

FIS1 Antibody Western Blot,Immunofluorescence validation (1:100 - 1:500 dilution) in RPE (Cat no:10956-1-AP)

FH

Siting (Verified Customer) (01-28-2020)

This antibody worked perfectly for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent/

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons

FH

Kishor (Verified Customer) (12-13-2018)

I got good results in both rat liver protein and H69 cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

FIS1 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:10956-1-AP)

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