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FIS1 Polyclonal antibody
FIS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10956-1-AP
|Positive WB detected in||HeLa cells, SH-SY5Y cells, pig brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue, mouse brain tissue, Jurkat cells, mouse spleen, rat spleen|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||rat brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hepa1-6 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
10956-1-AP targets FIS1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||bovine, Chinese hamster ovary (CHO) cell, hamster, human, mouse, pig, rat, squirrel|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FIS1 fusion protein Ag1409|
|Full Name||fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)|
|Calculated molecular weight||17 kDa|
|Observed molecular weight||17 kDa|
|GenBank accession number||BC009428|
|Gene ID (NCBI)||51024|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)
|Product Specific Protocols|
|WB protocol for FIS1 antibody 10956-1-AP||Download protocol|
|IHC protocol for FIS1 antibody 10956-1-AP||Download protocol|
|IF protocol for FIS1 antibody 10956-1-AP||Download protocol|
|IP protocol for FIS1 antibody 10956-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.
J Cell Physiol
Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.
Free Radic Biol Med
Low-dose oral copper treatment changes the hippocampal phosphoproteomic profile and perturbs mitochondrial function in a mouse model of Alzheimer's disease.
ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Megan (Verified Customer) (06-22-2021)
RPE cell over-expressing pcDNA hFis1. Note 1:500 was used here to detect over-expressed Fis1, but we also use 1:100 when detecting endogenous Fis1. Also successfully used to detect endogenous Fis1 levels via Western Blot (used @ 1:1000).
Siting (Verified Customer) (01-28-2020)
This antibody worked perfectly for western blot.
Chun (Verified Customer) (07-03-2019)
This antibody is excellent/
Kishor (Verified Customer) (12-13-2018)
I got good results in both rat liver protein and H69 cells.