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  • KD/KO Validated

FLCN Polyclonal antibody

FLCN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11236-2-AP

Synonyms

BHD, FLCL, FLCN, folliculin



Tested Applications

Positive WB detected inmouse thymus tissue, A431 cells, A549 cells, HeLa cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11236-2-AP targets FLCN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLCN fusion protein Ag1746
Full Name folliculin
Calculated molecular weight 38 kDa
Observed molecular weight 38-45 kDa, 64 kDa, 20-26 kDa
GenBank accession numberBC015687
Gene symbol FLCN
Gene ID (NCBI) 201163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma. This antibody can recognize all the 3 isoforms of FLCN (38-45 kDa,64 kDa,20-26 kDa).

Protocols

Product Specific Protocols
WB protocol for FLCN antibody 11236-2-APDownload protocol
IHC protocol for FLCN antibody 11236-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O?-methylguanine-induced apoptosis.

Authors - Sano Shiori S
  • KD Validated
humanIHC

Front Mol Biosci

FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma.

Authors - Xuyang Zhao
mouseWB

DNA Repair (Amst)

Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA.

Authors - Lim Teik How TH
  • KD Validated
mouseWB,IP

FASEB J

AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR.

Authors - Caterina Collodet