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FLCN Polyclonal antibody
FLCN Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11236-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse thymus tissue, A431 cells, A549 cells, HeLa cells, mouse kidney tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
IP | See 1 publications below |
Product Information
11236-2-AP targets FLCN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FLCN fusion protein Ag1746 |
Full Name | folliculin |
Calculated molecular weight | 38 kDa |
Observed molecular weight | 38-45 kDa, 64 kDa, 20-26 kDa |
GenBank accession number | BC015687 |
Gene symbol | FLCN |
Gene ID (NCBI) | 201163 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma. This antibody can recognize all the 3 isoforms of FLCN (38-45 kDa,64 kDa,20-26 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for FLCN antibody 11236-2-AP | Download protocol |
IHC protocol for FLCN antibody 11236-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochem Biophys Res Commun Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O?-methylguanine-induced apoptosis.
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Front Mol Biosci FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma. | ||
DNA Repair (Amst) Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA.
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FASEB J AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR. |