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- KD/KO Validated
FLCN Polyclonal antibody
FLCN Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11236-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HEK-293 cells, NCCIT cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
11236-2-AP targets FLCN in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FLCN fusion protein Ag1746 |
| Full Name | folliculin |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 64-75 kDa |
| GenBank Accession Number | BC015687 |
| Gene Symbol | FLCN |
| Gene ID (NCBI) | 201163 |
| RRID | AB_2103285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FLCN antibody 11236-2-AP | Download protocol |
| IHC protocol for FLCN antibody 11236-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR. | ||
DNA Repair (Amst) Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA.
| ||
Front Mol Biosci FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma. | ||
J Trace Elem Med Biol Methionine-chelated Zn promotes anabolism by integrating mTOR signal and autophagy pathway in juvenile yellow catfish. | ||
Biochem Biophys Res Commun Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O?-methylguanine-induced apoptosis.
| ||
Curr Res Neurobiol Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models |
