FLCN Polyclonal antibody

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  WB analysis using 11236-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11236-2-AP (FLCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 11236-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11236-2-AP (FLCN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FLCN Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11236-2-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

BHD, Birt-Hogg-Dube syndrome protein, FLCL, Folliculin

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, NCCIT cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11236-2-AP targets FLCN in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1746

  Product name: Recombinant human FLCN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-342 aa of BC015687

  Sequence: MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGDGNEDSPGQGEQAEEEEGGIQMNSRMRAHSPAEGASVESSSPGPKKSDMCEGCRSLAAGHPGYISHDKETSIKYVSHQHPSHPQLFSIVRQACVRSLSCEVCPGREGPIFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIITIMMDRIYLINSWPFLLGKVRGIIDELQGKALKVFEAEQFGCPQRAQRMNTAFTPFLHQRNGNAARSLTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEGAPTEDTLVQMEKLAGEAGVLLPGPWPGWPWGGTSCLLSWQESLREGNAALNQPRTSLREAHPPISV

• Full Name: folliculin
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 64-75 kDa
• GenBank Accession Number: BC015687
• Gene Symbol: FLCN
• Gene ID (NCBI): 201163
• RRID: AB_2103285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NFG4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IP	FASEB J; AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR.; Authors - Caterina Collodet
mouse	WB	DNA Repair (Amst); Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA.; Authors - Lim Teik How TH ; KD Validated
human	IHC	Front Mol Biosci; FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma.; Authors - Xuyang Zhao
	WB	J Trace Elem Med Biol; Methionine-chelated Zn promotes anabolism by integrating mTOR signal and autophagy pathway in juvenile yellow catfish.; Authors - Kun Wu
human	WB	Biochem Biophys Res Commun; Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O?-methylguanine-induced apoptosis.; Authors - Sano Shiori S ; KD Validated
rat	WB	Curr Res Neurobiol; Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models; Authors - Mira Seidel