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FLCN Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 11236-2-AP

Featured Product KD/KO validated

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Con: 47 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

FLCN Antibody WB HeLa cells 11236-2-AP

WB result of FLCN antibody (11236-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLCN transfected HeLa cells

FLCN Antibody WB mouse thymus tissue 11236-2-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11236-2-AP(FLCN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

FLCN Antibody IP HeLa cells 11236-2-AP

IP result of anti-FLCN(11236-2-AP) with Hela cell.

FLCN Antibody IHC human ovary tumor tissue 11236-2-AP

Immunohistochemical of paraffin-embedded human ovary tumor using 11236-2-AP(FLCN antibody) at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse thymus tissue, mouse kidney tissue, A549 cells, HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FLCN fusion protein Ag1746
Full name:
folliculin
Calculated molecular weight:
38 kDa
Observed molecular weight:
38-45 kDa,64 kDa,20-26 kDa
GenBank accession number:
BC015687
Gene ID (NCBI):
201163
Gene symbol
FLCN
Synonyms
BHD, FLCL, FLCN, folliculin

Background

FLCN, also named as BHD, belongs to the folliculin family. It may play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). FLCN may be a tumor suppressor. It is involved in colorectal tumorigenesis. It may be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. Mutation of FLCN may cause the BHD(Birt-Hogg-Dube syndrome ) or (PSP) primary spontaneous pneumothorax. And it may be involved in renal cell carcinoma. This antibody can recognize all the 3 isoforms of FLCN (38-45 kDa,64 kDa,20-26 kDa).


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