- Featured Product
- KD/KO Validated
FLI1 Polyclonal antibody
FLI1 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, ELISA
Cat no : 11347-1-AP
|Positive WB detected in||HL-60 cells, human brain tissue, Jurkat cells, mouse thymus tissue|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 4 publications below|
|IHC||See 1 publications below|
11347-1-AP targets FLI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FLI1 fusion protein Ag1899|
|Full Name||Friend leukemia virus integration 1|
|Calculated molecular weight||452 aa, 51 kDa|
|Observed molecular weight||51 kDa|
|GenBank accession number||BC010115|
|Gene ID (NCBI)||2313|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.
|Product Specific Protocols|
|WB protocol for FLI1 antibody 11347-1-AP||Download protocol|
|IHC protocol for FLI1 antibody 11347-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Suppression of Fli-1 protects against pericyte loss and cognitive deficits in Alzheimer's disease.
Mol Ther Nucleic Acids
circCAMSAP1 promotes osteosarcoma progression and metastasis by sponging miR-145-5p and regulating FLI1 expression.
Front Cell Infect Microbiol
Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.
Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene.
Mol Med Rep
Fli‑1 promotes metastasis by regulating MMP2 signaling in hepatocellular carcinoma.