• Featured Product
  • KD/KO Validated

FLI1 Polyclonal antibody

FLI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11347-1-AP

Synonyms

EWSR2, FLI1, Proto oncogene Fli 1, SIC 1, Transcription factor ERGB



Tested Applications

Positive WB detected inHL-60 cells, mouse thymus tissue, human brain tissue, Jurkat cells, MOLT-4 cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11347-1-AP targets FLI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLI1 fusion protein Ag1899
Full Name Friend leukemia virus integration 1
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC010115
Gene symbol FLI1
Gene ID (NCBI) 2313
RRIDAB_2106102
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.

Protocols

Product Specific Protocols
WB protocol for FLI1 antibody 11347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Mol Ther

Suppression of Fli-1 protects against pericyte loss and cognitive deficits in Alzheimer's disease.

Authors - Pengfei Li
humanIHC

Mol Ther Nucleic Acids

circCAMSAP1 promotes osteosarcoma progression and metastasis by sponging miR-145-5p and regulating FLI1 expression.

Authors - Zizheng Chen
humanWB

Front Cell Infect Microbiol

Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.

Authors - Fengxue Geng
humanWB

Cancer

Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene.

Authors - Zhang Jianjun J
  • KD Validated
humanWB

Mol Med Rep

Fli‑1 promotes metastasis by regulating MMP2 signaling in hepatocellular carcinoma.

Authors - Huiling Wang
  • KD Validated