Tested Applications
| Positive WB detected in | PC-3 cells, mouse liver tissue, DU 145 cells, LNCaP cells, VCaP cells, RAW 264.7 cells, MCF-7 cells, rat liver tissue |
| Positive IHC detected in | human liver tissue, mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16864-1-AP targets FMO5-specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | flavin containing monooxygenase 5 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_001461 |
| Gene Symbol | FMO5 |
| Gene ID (NCBI) | 2330 |
| RRID | AB_11125955 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49326 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FMO5-specific antibody 16864-1-AP | Download protocol |
| IF protocol for FMO5-specific antibody 16864-1-AP | Download protocol |
| IHC protocol for FMO5-specific antibody 16864-1-AP | Download protocol |
| WB protocol for FMO5-specific antibody 16864-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FMO5-specific Polyclonal antibody (Cat# 16864-1-AP) has 2 total citations. They appear in Journal of Translational Medicine and Drug Metabolism and Disposition. The research fields include diabetic kidney disease with amino acid metabolism regulation, and glucose homeostasis with gut bacteria sensing. Both studies used mouse models, applying the antibody for IF, WB, and IHC.
| Species | Application | Title |
|---|---|---|
J Transl Med Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism | ||
Drug Metab Dispos Identification of flavin-containing monooxygenase 5 (FMO5) as a regulator of glucose homeostasis and a potential sensor of gut bacteria.
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