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  • KD/KO Validated

FMO5-specific Polyclonal antibody

FMO5-specific Polyclonal Antibody for WB, IHC, ELISA, IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16864-1-AP


FMO5, Flavin-containing monooxygenase 5, EC:, EC:, EC:1.14.13.-

Tested Applications

Positive WB detected inPC-3 cells, mouse liver tissue, DU 145 cells, LNCaP cells, VCaP cells, RAW 264.7 cells, MCF-7 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16864-1-AP targets FMO5-specific in WB, IHC, ELISA, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name flavin containing monooxygenase 5
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_001461
Gene Symbol FMO5
Gene ID (NCBI) 2330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 3 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for FMO5-specific antibody 16864-1-APDownload protocol
IHC protocol for FMO5-specific antibody 16864-1-APDownload protocol
IF protocol for FMO5-specific antibody 16864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Drug Metab Dispos

Identification of flavin-containing monooxygenase 5 (FMO5) as a regulator of glucose homeostasis and a potential sensor of gut bacteria.

Authors - Flora Scott
  • KO Validated

J Transl Med

Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism

Authors - Yi-Xi Li