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Fibronectin Polyclonal antibody
Fibronectin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15613-1-AP
Validation Data Gallery
|Positive WB detected in||human plasma, NIH/3T3 cells, MDA-MB-453s cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, rat bladder tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15613-1-AP targets Fibronectin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, medaka embryos, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fibronectin fusion protein Ag8004|
|Full Name||fibronectin 1|
|Calculated molecular weight||2386 aa, 263 kDa|
|Observed molecular weight||250-275 kDa|
|GenBank accession number||BC005858|
|Gene ID (NCBI)||2335|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fibronectins play a role in cell adhesion, motility, wound healing, and the maintenance of cell shape. Fibronectins bind to cell surfaces via interactions with integrins and various molecules such as collagen, fibrin, heparin, DNA, and actin (PMID: 3326130). Cellular interaction with fibronectin (FN1) promotes cell cycle progression and proliferation by influencing cyclins.
What is the molecular weight of FN1?
The molecular weight of FN1 is 220 kDa.
What is the tissue specificity of FN1?
FN1 is a large glycoprotein that exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1) (PMID: 21923916). Plasma FN1, or the dimeric form, is secreted by hepatocytes, whereas cellular FN1, the dimeric or cross-linked multimeric form, is produced by fibroblasts and epithelial cells and deposited as fibrils in the extracellular matrix.
What are the post-translational modifications of FN1?
FN1 is often sulfated and its C-terminal NC1 peptide, anastellin, is produced as a result of proteolytic processing and can inhibit tumor growth, angiogenesis, and metastasis by activating p38 MAPK and inhibiting lysophospholipid signaling (PMID: 11209058).
What is FN1's role in embryogenesis?
Fibronectin has a crucial role in the development of the left-right body axis during embryogenesis (PMID: 21466802).
What is FN1's involvement in disease?
Fibronectin glomerulopathy is a kidney disease that eventually leads to end-stage renal disease and is caused by mutations in the FN1 gene. Mutations in FN1 lead to the production of abnormal fibronectin protein that is deposited in the glomeruli of the kidney (PMID: 18268355).
|Product Specific Protocols|
|WB protocol for Fibronectin antibody 15613-1-AP||Download protocol|
|IHC protocol for Fibronectin antibody 15613-1-AP||Download protocol|
|IF protocol for Fibronectin antibody 15613-1-AP||Download protocol|
|IP protocol for Fibronectin antibody 15613-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Susanne (Verified Customer) (08-23-2022)
under reduced conditions, blocked with 5% milk powder, incubation over night 4°C
Pankhuri (Verified Customer) (08-19-2022)
I tried this antibody for IF in neural stem cells. It works great in the conc. 1:200.
Ren Jie (Verified Customer) (07-11-2022)
Very clean bands near 220 kD indicating fibronectin monomer
SD (Verified Customer) (07-08-2022)
zunyi (Verified Customer) (03-11-2022)
This ab is used for immunohistochemistry on mouse paraffin-embedded sections. Antigen-retrieve, using pH 6.0 citric buffer, was performed prior to staining.
Lindsey (Verified Customer) (05-24-2021)
Works beautifully! Looking forward to working more with this antibody in different tissue/prep types