FOXA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FOXA2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 22474-1-AP

Synonyms

forkhead box A2, Forkhead box protein A2, FOXA2, HNF 3 beta, HNF 3B, HNF3B, TCF 3B, TCF3B, Transcription factor 3B

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Validation Data Gallery

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  WB analysis of A549 using 22474-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22474-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22474-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 22474-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22474-1-AP

  IP Result of anti-FOXA2 (IP:22474-1-AP, 3ug; Detection:22474-1-AP 1:300) with HepG2 cells lysate 1920ug.

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  IHC staining of human liver cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat stomach using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22474-1-AP (FOXA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 22474-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 22474-1-AP (FOXA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, PC-13 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: rat stomach tissue, human colon cancer tissue, human liver cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 22474-1-AP is FOXA2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXA2 fusion protein Ag18221
• Full Name: forkhead box A2
• Calculated molecular weight: 457 aa, 48 kDa
• Observed molecular weight: 55-65 kDa
• GenBank accession number: BC011780
• Gene symbol: FOXA2
• Gene ID (NCBI): 3170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

Publications

Species	Application	Title
human	chIP	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
human	IF	Chemosphere; Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells.; Authors - Yujie Liu
human	WB,IHC,IF	Am J Cancer Res; Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.; Authors - Zhanjun Chen ; KD Validated
human	WB	Int J Biol Sci; EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways.; Authors - Lin Yang
mouse	WB	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
human	IF	Ann Transl Med; Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.; Authors - Sang Luo