|Positive WB detected in||A549 cells, HepG2 cells, PC-13 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human pancreas tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22474-1-AP is FOXA2 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FOXA2 fusion protein Ag18221|
|Full Name||forkhead box A2|
|Calculated molecular weight||457 aa, 48 kDa|
|Observed molecular weight||55-65 kDa|
|GenBank accession number||BC011780|
|Gene ID (NCBI)||3170|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.
|Product Specific Protocols|
|WB protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|IHC protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|IF protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|IP protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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