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  • KD/KO Validated

FOXA2 Polyclonal antibody

FOXA2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 22474-1-AP

Synonyms

forkhead box A2, Forkhead box protein A2, FOXA2, HNF 3 beta, HNF 3B, HNF3B, TCF 3B, TCF3B, Transcription factor 3B



Tested Applications

Positive WB detected inHT-29 cells, A549 cells, HepG2 cells, MDA-MB-231 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22474-1-AP targets FOXA2 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXA2 fusion protein Ag18221
Full Name forkhead box A2
Calculated molecular weight 457 aa, 48 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC011780
Gene symbol FOXA2
Gene ID (NCBI) 3170
RRIDAB_2879110
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

Protocols

Product Specific Protocols
WB protocol for FOXA2 antibody 22474-1-APDownload protocol
IHC protocol for FOXA2 antibody 22474-1-APDownload protocol
IP protocol for FOXA2 antibody 22474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanchIP

Cell Res

Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.

Authors - Shuaishuai Teng
humanIF

Chemosphere

Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells.

Authors - Yujie Liu
humanWB,IHC,IF

Am J Cancer Res

Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.

Authors - Zhanjun Chen
  • KD Validated
humanWB

Int J Biol Sci

EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways.

Authors - Lin Yang
mouseWB

Mol Cell Endocrinol

Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.

Authors - Xinghong Guo
humanIF

Ann Transl Med

Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.

Authors - Sang Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
  • Cell Tissue Type: Mouse Liver tissue
FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
  • Cell Tissue Type: Mouse Liver tissue