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FOXA2 Polyclonal antibody
FOXA2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, chIP, ELISA
Conjugate
Unconjugated
Cat no : 22474-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HepG2 cells, PC-13 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | rat stomach tissue, human colon cancer tissue, human liver cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IHC | See 3 publications below |
IF | See 6 publications below |
ChIP | See 1 publications below |
Product Information
The immunogen of 22474-1-AP is FOXA2 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FOXA2 fusion protein Ag18221 |
Full Name | forkhead box A2 |
Calculated molecular weight | 457 aa, 48 kDa |
Observed molecular weight | 55-65 kDa |
GenBank accession number | BC011780 |
Gene symbol | FOXA2 |
Gene ID (NCBI) | 3170 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.
Protocols
Product Specific Protocols | |
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WB protocol for FOXA2 antibody 22474-1-AP | Download protocol |
IHC protocol for FOXA2 antibody 22474-1-AP | Download protocol |
IF protocol for FOXA2 antibody 22474-1-AP | Download protocol |
IP protocol for FOXA2 antibody 22474-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer. | ||
Chemosphere Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells. | ||
Am J Cancer Res Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.
| ||
Int J Biol Sci EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways. | ||
Mol Cell Endocrinol Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells. | ||
Ann Transl Med Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro. |