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  • KD/KO Validated

FOXA2 Polyclonal antibody

FOXA2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 22474-1-AP

Synonyms

forkhead box A2, Forkhead box protein A2, FOXA2, HNF 3 beta, HNF 3B, HNF3B, TCF 3B, TCF3B, Transcription factor 3B



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, PC-13 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22474-1-AP is FOXA2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXA2 fusion protein Ag18221
Full Name forkhead box A2
Calculated molecular weight 457 aa, 48 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC011780
Gene symbol FOXA2
Gene ID (NCBI) 3170
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

Protocols

Product Specific Protocols
WB protocol for FOXA2 antibody 22474-1-APDownload protocol
IHC protocol for FOXA2 antibody 22474-1-APDownload protocol
IF protocol for FOXA2 antibody 22474-1-APDownload protocol
IP protocol for FOXA2 antibody 22474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer.

Authors - Bing Chen
humanIF

Cell Death Dis

Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling.

Authors - Minjing Ke
humanIHC

Biochem Biophys Res Commun

Early prediction of the differentiation potential during the formation of human iPSC-derived embryoid bodies.

Authors - Ning-Ning Guo
humanWB

Int J Biol Sci

EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways.

Authors - Lin Yang
humanWB,IHC,IF

Am J Cancer Res

Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.

Authors - Zhanjun Chen
  • KD Validated
humanIF

Chemosphere

Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells.

Authors - Yujie Liu