FOXA2 Polyclonal antibody

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  WB analysis using 22474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22474-1-AP

  WB result of FOXA2 antibody (22474-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and sg-FOXA2 transfected HepG2 cells.

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  IP experiment of HepG2 using 22474-1-AP

  IP result of anti-FOXA2 (IP:22474-1-AP, 4ug; Detection:22474-1-AP 1:500) with HepG2 cells lysate 1320 ug.

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  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22474-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat stomach using 22474-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat stomach tissue using FOXA2 antibody (22474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

FOXA2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 22474-1-AP

Publications(23)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, chIP, ELISA

Synonyms

forkhead box A2, Forkhead box protein A2, Hepatocyte nuclear factor 3-beta, HNF 3 beta, HNF 3B

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Tested Applications

• Positive WB detected in: HT-29 cells, HepG2 cells, A549 cells, MDA-MB-231 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

22474-1-AP targets FOXA2 in WB, IHC, IF-P, IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXA2 fusion protein Ag18221
• Full Name: forkhead box A2
• Calculated Molecular Weight: 457 aa, 48 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC011780
• Gene Symbol: FOXA2
• Gene ID (NCBI): 3170
• RRID: AB_2879110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y261
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

Publications

Species	Application	Title
human	chIP	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
human	WB	iScience; NatD epigenetically activates FOXA2 expression to promote breast cancer progression by facilitating MMP14 expression; Authors - Mengying Xing
human	IF	Chemosphere; Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells.; Authors - Yujie Liu
human	WB,IHC,IF	Am J Cancer Res; Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.; Authors - Zhanjun Chen ; KD Validated
human	WB	Int J Biol Sci; EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways.; Authors - Lin Yang
mouse	WB	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue

FH

Kamal (Verified Customer) (07-05-2023)

Worked when diluted in TBST for WB and 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue