Validation Data Gallery
|Positive WB detected in||A549 cells, HepG2 cells, PC-13 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||rat stomach tissue, human colon cancer tissue, human liver cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22474-1-AP is FOXA2 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FOXA2 fusion protein Ag18221|
|Full Name||forkhead box A2|
|Calculated molecular weight||457 aa, 48 kDa|
|Observed molecular weight||55-65 kDa|
|GenBank accession number||BC011780|
|Gene ID (NCBI)||3170|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.
|Product Specific Protocols|
|WB protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|IHC protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|IF protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|IP protocol for FOXA2 antibody 22474-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Upregulated forkhead-box A3 elevates the expression of forkhead-box A1 and forkhead-box A2 to promote metastasis in esophageal cancer.
Cell Death Dis
Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling.
Arab J Gastroenterol
Forkhead box A2 transcriptionally activates hsa-let-7 g to inhibit hypoxia-induced epithelial-mesenchymal transition by targeting c14orf28 in colorectal cancer.
Biochem Biophys Res Commun
Early prediction of the differentiation potential during the formation of human iPSC-derived embryoid bodies.
Ann Transl Med
Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.
Mol Cell Endocrinol
Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.