FSHR Polyclonal antibody

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  WB analysis using 22665-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22665-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat ovary using 22665-1-AP

  rat ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 22665-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 22665-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 22665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 22665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

FSHR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22665-1-AP

Publications(79)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, ELISA

Synonyms

LGR1, FSHRO, FSH-R, FSH R, Follitropin receptor

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Tested Applications

• Positive WB detected in: mouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 55 publications below
• IHC: See 17 publications below

Product Information

22665-1-AP targets FSHR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep, goat, cat, ovis aries (sheep)
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FSHR fusion protein Ag18158
• Full Name: follicle stimulating hormone receptor
• Calculated Molecular Weight: 695 aa, 78 kDa
• Observed Molecular Weight: 71-78 kDa
• GenBank Accession Number: BC118548
• Gene Symbol: FSHR
• Gene ID (NCBI): 2492
• RRID: AB_2631204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23945
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
human,mouse	WB	Autophagy; Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1; Authors - Tong Shao
human,mouse	WB,IHC	Theranostics; FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation; Authors - Xingyan Xu
mouse	WB	J Adv Res; Core fucosylation regulates the ovarian response via FSH receptor during follicular development; Authors - Tiantong Wang
human		Adv Healthc Mater; 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization; Authors - Meghan A Robinson
mouse	IHC	Redox Biol; Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice.; Authors - Shotaro Hayashi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nolan (Verified Customer) (04-11-2022)

Antibody works better if you stain at room temperature for 2 hours instead of 4C overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse FSHR