FUCA1 Monoclonal antibody

FUCA1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G5C5

Cat no : 67251-1-Ig

Synonyms

Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67251-1-Ig targets FUCA1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FUCA1 fusion protein Ag9638
Full Name fucosidase, alpha-L- 1, tissue
Calculated molecular weight 461 aa, 53 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC017338
Gene symbol FUCA1
Gene ID (NCBI) 2517
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).

Protocols

Product Specific Protocols
WB protocol for FUCA1 antibody 67251-1-IgDownload protocol
IHC protocol for FUCA1 antibody 67251-1-IgDownload protocol
IF protocol for FUCA1 antibody 67251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols