FUCA1 Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67251-1-Ig (same clone as 67251-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67251-1-Ig (same clone as 67251-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67251-1-Ig (same clone as 67251-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

FUCA1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 67251-1-PBS

Clone No.1G5C5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

FUCA, EC:3.2.1.51, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase I, Alpha-L-fucosidase 1

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Product Information

67251-1-PBS targets FUCA1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9638

  Product name: Recombinant human FUCA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 113-461 aa of BC017338

  Sequence: EEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK

• Full Name: fucosidase, alpha-L- 1, tissue
• Calculated Molecular Weight: 461 aa, 53 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC017338
• Gene Symbol: FUCA1
• Gene ID (NCBI): 2517
• RRID: AB_2882528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04066
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).