FUK Polyclonal antibody

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  WB analysis using 13541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13541-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 13541-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13541-1-AP (FUK Antibody) at dilution of 1:50 (under 40x lens).

FUK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13541-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

1110046B12Rik, fucokinase, FUK, L fucose kinase

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Tested Applications

• Positive WB detected in: MCF-7 cells, BxPC-3 cells, mouse thymus tissue, mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13541-1-AP targets FUK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4481

  Product name: Recombinant human FUK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 695-1084 aa of BC032542

  Sequence: TEQVELPGPGQWVVAECPARVDFSGGWSDTPPLAYELGGAVLGLAVRVDGRRPIGARARRIPEPELWLAVGPRQDEMTVKIVCRCLADLRDYCQPHAPGALLKAAFICAGIVHVHSELQLSEQLLRTFGGGFELHTWSELPHGSGLGTSSILAGTALAALQRAAGRVVGTEALIHAVLHLEQVLTTGGGWQDQVGGLMPGIKVGRSRAQLPLKVEVEEVTVPEGFVQKLNDHLLLVYTGKTRLARNLLQDVLRSWYARLPAVVQNAHSLVRQTEECAEGFRQGSLPLLGQCLTSYWEQKKLMAPGCEPLTVRRMMDVLAPHVHGQSLAGAGGGGFLYLLTKEPQQKEALEAVLAKTEGLGNYSIHLVEVDTQGLSLKLLGTEASTCCPFP

• Full Name: fucokinase
• Calculated Molecular Weight: 1084 aa, 118 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC032542
• Gene Symbol: FUK
• Gene ID (NCBI): 197258
• RRID: AB_2104961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N0W3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUK, also named FCSK, belongs to the GHMP kinase family. FUK encodes fucokinase, the only enzyme capable of converting L-fucose to fucose-1-phosphate, which will ultimately be used for synthesizing GDP-fucose, the donor substrate for all fucosyltransferases (PMID: 30503518).