Frizzled 2 Polyclonal antibody

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  WB analysis using 24272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 24272-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 24272-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 24272-1-AP (Frizzled 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 24272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24272-1-AP (Frizzled 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24272-1-AP (Frizzled 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Frizzled 2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24272-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

FZD2, Frizzled-2, Fz 2, Fz-2, FzE2

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Tested Applications

• Positive WB detected in: Caco-2 cells, mouse colon tissue, rat kidney tissue, HEK-293 cells, mouse colon tisue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 1 publications below

Product Information

24272-1-AP targets Frizzled 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Frizzled 2 fusion protein Ag18003
• Full Name: frizzled homolog 2 (Drosophila)
• Calculated Molecular Weight: 565 aa, 64 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC113402
• Gene Symbol: Frizzled 2
• Gene ID (NCBI): 2535
• RRID: AB_2879463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14332
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Frizzled 2(FZD2) also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. FZD2 is an important receptor in the Wnt pathway, which is highly expressed in malignant tumors and helps regulate multiple tumor behaviors. Its expression level is related to prognosis. Moreover, high level of FZD2 had significant correlation with poor prognosis in Breast cancer (BC) patients(PMID: 33832493).

Publications

Species	Application	Title
mouse	WB	Biol Res; Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model; Authors - Yanyi Ding
human	WB	Front Microbiol; Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.; Authors - Dennis Schöttelndreier
rat	WB	Pharm Biol; Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.; Authors - Ying Xu
mouse	WB	Evid Based Complement Alternat Med; Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.; Authors - Xuesong Yang
human	WB	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu ; KD Validated
human	WB	Genes Dis; Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway.; Authors - Yi Liu