DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Recombinant protein and More (4)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E7B4

Cat no : 66008-2-Ig

Synonyms



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66008-2-Ig targets DDDDK tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Cited Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, theDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This DDDDK-tag antibody can be used to detect the DDDDK tagged protein with pCMV-DDDDK, p3XFLAG-CMV, pcDNA3.1-DDDDK, pef-NEO-DDDDK, DDDDK-RIG-I, pLenti6-3XDDDDK and some other vectors.

Publications

SpeciesApplicationTitle
humanCoIP

Autophagy

YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization.

Authors - Yang Xu
WB

Nat Commun

Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.

Authors - Ying Shen

Cell Rep

Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.

Authors - Hao Zhou

J Exp Clin Cancer Res

Shisa3 brakes resistance to EGFR-TKIs in lung adenocarcinoma by suppressing cancer stem cell properties.

Authors - Jiahui Si
IP

Oncotarget

Variant 2 of KIAA0101, antagonizing its oncogenic variant 1, might be a potential therapeutic strategy in hepatocellular carcinoma.

Authors - Lijuan Liu
WB

Clin Epigenetics

Methylation of ZNF331 is an independent prognostic marker of colorectal cancer and promotes colorectal cancer growth.

Authors - Yuzhu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yijun (Verified Customer) (01-01-2020)

This tag antibody is with low specificity. Even the samples is not transfected at all (Empty), you will get a band.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293FT, PC3, LNCaP
DDDDK tag Antibody Western Blot, validation (1:1000 dilution) in HEK293FT, PC3, LNCaP (Cat no:66008-2-Ig)
FH

David (Verified Customer) (08-07-2019)

Protein in bacterial expression system with Flag-tag. We ran a chemi blot using anti mouse secondary.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Bacteria
DDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacteria (Cat no:66008-2-Ig)