DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DDDDK tag Monoclonal Antibody for ELISA
Cat no : 66008-2-Ig
|Sample-dependent, check data in validation data gallery|
66008-2-Ig targets DDDDK tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.
|Tested Reactivity||Recombinant protein|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Full Name||DDDDK tag|
|Gene ID (NCBI)|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, theDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This DDDDK-tag antibody can be used to detect the DDDDK tagged protein with pCMV-DDDDK, p3XFLAG-CMV, pcDNA3.1-DDDDK, pef-NEO-DDDDK, DDDDK-RIG-I, pLenti6-3XDDDDK and some other vectors.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yijun (Verified Customer) (01-01-2020)
This tag antibody is with low specificity. Even the samples is not transfected at all (Empty), you will get a band.
David (Verified Customer) (08-07-2019)
Protein in bacterial expression system with Flag-tag. We ran a chemi blot using anti mouse secondary.