DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DDDDK tag Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Recombinant protein and More (4)
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1E7B4
Cat no : 66008-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 17 publications below |
| IF | See 2 publications below |
| IP | See 6 publications below |
| CoIP | See 3 publications below |
Product Information
66008-2-Ig targets DDDDK tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.
Background Information
DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, theDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This DDDDK-tag antibody can be used to detect the DDDDK tagged protein with pCMV-DDDDK, p3XFLAG-CMV, pcDNA3.1-DDDDK, pef-NEO-DDDDK, DDDDK-RIG-I, pLenti6-3XDDDDK and some other vectors.
Publications
| Species | Application | Title |
|---|---|---|
Autophagy YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization. | ||
Nat Commun Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. | ||
Cell Rep Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis. | ||
J Exp Clin Cancer Res Shisa3 brakes resistance to EGFR-TKIs in lung adenocarcinoma by suppressing cancer stem cell properties. | ||
Oncotarget Variant 2 of KIAA0101, antagonizing its oncogenic variant 1, might be a potential therapeutic strategy in hepatocellular carcinoma. | ||
Clin Epigenetics Methylation of ZNF331 is an independent prognostic marker of colorectal cancer and promotes colorectal cancer growth. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yijun (Verified Customer) (01-01-2020) | This tag antibody is with low specificity. Even the samples is not transfected at all (Empty), you will get a band.
 |
FH David (Verified Customer) (08-07-2019) | Protein in bacterial expression system with Flag-tag. We ran a chemi blot using anti mouse secondary.
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