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Flag tag Antibody 23 Publications

Mouse Monoclonal| Catalog number: 66008-2-Ig |CloneNo.: 1E7B4

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

Flag tag Antibody IP Recombinant protein protein 66008-2-Ig

IP Result of anti-Flag tag (IP:66008-2-Ig, 3ug; Detection:20543-1-AP 1:2000) with Recombinant protein protein lysate 1280ug.

Flag tag Antibody WB Transfected HEK-293 cells 66008-2-Ig

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-2-Ig( Flag tag Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Flag tag Antibody WB ag19172 66008-2-Ig

Recombinant protein were Recombinant protein were subjected to SDS PAGE followed by western blot with 66008-2-Ig (Flag tag Antibody) at various dilutions incubated at room temperature for 1.5 hours

Flag tag Antibody IF Transfected HEK-293 cells 66008-2-Ig

Immunofluorescent analysis of Transfected HEK-293 cells using 66008-2-Ig(Flag tag antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Recombinant protein, human, mouse, rat
Positive WB detected in:
Transfected HEK-293 cells, ag19172
Positive IP detected in:
Recombinant protein protein
Positive IF detected in:
Transfected HEK-293 cells
Recommended dilution:
WB : 1:1000-1:10000
IP : 0.5-4.0 ug for IP and 1:10-1:100 for WB
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
Flag tag
Calculated molecular weight:
Observed molecular weight:
GenBank accession number:
Gene ID (NCBI):
Gene symbol
Synonyms
DDK tag, DYKDDDDK, FLAG, Flag tag

Background

Flag tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of Flag tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the Flag tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with Flag tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This Flag-tag antibody can be used to detect the FLAGGED protein with pCMV-FLAG, p3XFLAG-CMV, pcDNA3.1-FLAG, pef-NEO-FLAG, FLAG-RIG-I, pLenti6-3XFLAG and some other vectors.


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