DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Recombinant protein and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E7B4

Cat no : 66008-2-Ig

Synonyms

DDDDK tag


"DDDDK tag Antibodies" Comparison

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Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66008-2-Ig targets DDDDK tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, theDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This DDDDK-tag antibody can be used to detect the DDDDK tagged protein with pCMV-DDDDK, p3XFLAG-CMV, pcDNA3.1-DDDDK, pef-NEO-DDDDK, DDDDK-RIG-I, pLenti6-3XDDDDK and some other vectors.

Publications

SpeciesApplicationTitle
WB,IP

Circ Res

Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction.

Authors - Guizhen Zhao
CoIP,WB

Nat Commun

Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet.

Authors - Song Liu
WB

Nat Commun

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Authors - Hu Lei
WB

Bone Res

TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling.

Authors - Wanlei Yang

Nucleic Acids Res

The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.

Authors - Huiwu Ouyang

Autophagy

Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.

Authors - M Iqbal Hossain

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yijun (Verified Customer) (01-01-2020)

This tag antibody is with low specificity. Even the samples is not transfected at all (Empty), you will get a band.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293FT, PC3, LNCaP
DDDDK tag Antibody Western Blot, validation (1:1000 dilution) in HEK293FT, PC3, LNCaP (Cat no:66008-2-Ig)
FH

David (Verified Customer) (08-07-2019)

Protein in bacterial expression system with Flag-tag. We ran a chemi blot using anti mouse secondary.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Bacteria
DDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacteria (Cat no:66008-2-Ig)