DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b


Recombinant protein and More (2)







Cat no : 66008-2-Ig



"DDDDK tag Antibodies" Comparison

View side-by-side comparison of DDDDK tag antibodies from other vendors to find the one that best suits your research needs.

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66008-2-Ig targets DDDDK tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DDDDK tag
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, theDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This DDDDK-tag antibody can be used to detect the DDDDK tagged protein with pCMV-DDDDK, p3XFLAG-CMV, pcDNA3.1-DDDDK, pef-NEO-DDDDK, DDDDK-RIG-I, pLenti6-3XDDDDK and some other vectors.



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Yijun (Verified Customer) (01-01-2020)

This tag antibody is with low specificity. Even the samples is not transfected at all (Empty), you will get a band.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293FT, PC3, LNCaP
DDDDK tag Antibody Western Blot, validation (1:1000 dilution) in HEK293FT, PC3, LNCaP (Cat no:66008-2-Ig)

David (Verified Customer) (08-07-2019)

Protein in bacterial expression system with Flag-tag. We ran a chemi blot using anti mouse secondary.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Bacteria
DDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacteria (Cat no:66008-2-Ig)