CoraLite®488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b


Recombinant protein




CoraLite®488 Fluorescent Dye



Cat no : CL488-66008


DYKDDDDK, Flag tag

Tested Applications

Positive IF detected inTransfected HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66008-1-Ig

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66008 targets DYKDDDDK in IF applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.


Product Specific Protocols
IF protocol for CL488 DYKDDDDK antibody CL488-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols