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DDDDK tag antibody (Equivalent To FLAG® Antibodies) 5 Publications

Mouse Monoclonal| Catalog number: 66008-3-Ig |CloneNo.: 2B3C4

3 customer reviews

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Freight/Packing

Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

DDDDK tag Antibody WB HEK-293 cells 66008-3-Ig

HEK-293 and DDDDK-DPM1 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-3-Ig (DDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

DDDDK tag Antibody IP Transfected HEK-293 cells 66008-3-Ig

IP result of anti-DDDDK tag(IP:66008-3-Ig, 5ug; Detection:66008-3-Ig 1:1000) with Transfected HEK-293 cells lysate 1600 ug

DDDDK tag Antibody IF Transfected HEK-293 cells 66008-3-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed Transfected HEK-293 cells using 66008-3-Ig (DDDDK tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
recombinant protein
Positive WB detected in:
Transfected HEK-293 cells
Positive IP detected in:
Transfected HEK-293 cells
Positive IF detected in:
Transfected HEK-293 cells
Recommended dilution:
WB : 1:1000-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:200-1:800
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
DDDDK tag
Calculated molecular weight:
Observed molecular weight:
GenBank accession number:
Gene ID (NCBI):
Gene symbol
Synonyms
DDDDK tag, DYKDDDDK

Background

DDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DDDDK Tag Antibody is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

DDDDK tag Mouse Monoclonal antibody

Chun (Verified Customer), 12-05-2019
Applications
Western Blot,Immunohistochemistry,Immunofluorescence,
Primary Antibody Dilution
1:200 ~1: 2,000
No image submitted with review

No image submitted with review

Cell/Tissue Type
HEK293 and HeLa cells
Lot Number
Excellent antibody

DDDDK tag Mouse Monoclonal antibody

David (Verified Customer), 08-07-2019
Applications
Western Blot,
Primary Antibody Dilution
1/1000
DDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacterial (Cat no:66008-3-Ig)

DDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacterial (Cat no:66008-3-Ig)

Cell/Tissue Type
Bacterial
Lot Number
Bacterial Expression system with Flag tag. This seems to be very similar to the other mouse flag tag that we bought before. This works great and we got good results.

DDDDK tag Mouse Monoclonal antibody

Daniela (Verified Customer), 06-03-2019
Applications
Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture,
Primary Antibody Dilution
1:1000
DDDDK tag Antibody Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture, validation (1:1000 dilution) in HeLa cells (Cat no:66008-3-Ig)

DDDDK tag Antibody Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture, validation (1:1000 dilution) in HeLa cells (Cat no:66008-3-Ig)

Cell/Tissue Type
HeLa cells
Lot Number
In the figure, immunoblot of total cell lysate of HeLa cells transfected (left lane) with recombinant DNA expressing for a protein with the DDDDK-tag at its C-terminus. In the right lane, total cell lysate of HeLa moc transfection. Per each lane, 30ug of protein were loaded.

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