DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (1)

Applications

WB, RIP, IP, IHC, IF, CoIP

Conjugate

Unconjugated

CloneNo.

2B3C4

Cat no : 66008-3-Ig

Synonyms

DDDDK tag, DYKDDDDK


"DYKDDDDK tag Antibodies" Comparison

View side-by-side comparison of DYKDDDDK tag antibodies from other vendors to find the one that best suits your research needs.
  • WB analysis of HEK-293 using 66008-3-Ig

    WB analysis of HEK-293 using 66008-3-Ig

    HEK-293 and DDDDK-DPM1 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-3-Ig (DDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of Transfected HEK-293 using 66008-3-Ig

    IP experiment of Transfected HEK-293 using 66008-3-Ig

    IP result of anti-DDDDK tag (IP:66008-3-Ig, 5ug; Detection:66008-3-Ig 1:1000) with Transfected HEK-293 cells lysate 1600 ug.

  • IF Staining of Transfected HEK-293 using 66008-3-Ig

    IF Staining of Transfected HEK-293 using 66008-3-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66008-3-Ig (DDDDK tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).


Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66008-3-Ig targets DYKDDDDK tag in WB, RIP, IP, IHC, IF, CoIP applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 66008-3-IgDownload protocol
IF protocol for DYKDDDDK tag antibody 66008-3-IgDownload protocol
IP protocol for DYKDDDDK tag antibody 66008-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Fish Shellfish Immunol

Identification and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in common Chinese cuttlefish Sepiella japonica.

Authors - Zan Pang
WB,IP

J Neurochem

Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.

Authors - Robert J Andrew

Cell Death Dis

SPOP suppresses pancreatic cancer progression by promoting the degradation of NANOG.

Authors - Peng Tan
WB

Mol Genet Genomic Med

A novel homozygous nonsense ZP1 variant causes human female infertility associated with empty follicle syndrome (EFS).

Authors - Qianhua Xu

Cell Death Dis

FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation.

Authors - Xiao Liu
WB

Cell Death Dis

PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53.

Authors - Wuyi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (11-10-2021)

Very clean detection of overexpressed FLAG tagged protein in HEK293T cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Transfected HEK293T cell line
FH

Jinwei (Verified Customer) (09-20-2021)

DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-3-Ig) works fine.

  • Applications: Western Blot
  • Cell Tissue Type: HEK293
DYKDDDDK tag Antibody Western Blot validation ( dilution) in HEK293 (Cat no:66008-3-Ig)
FH

Chun (Verified Customer) (09-07-2020)

This is an excellent antibody for both IF and IB.

  • Applications: Western Blot,Immunofluorescence
  • Primary Antibody Dilution: 1:200 (IF)~2,000 (IB)
  • Cell Tissue Type: HeLa and HEK293 cells
FH

Chun (Verified Customer) (12-05-2019)

Excellent antibody

  • Applications: Western Blot,Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:200 ~1: 2,000
  • Cell Tissue Type: HEK293 and HeLa cells
FH

David (Verified Customer) (08-07-2019)

Bacterial Expression system with Flag tag. This seems to be very similar to the other mouse flag tag that we bought before. This works great and we got good results.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Bacterial
DYKDDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacterial (Cat no:66008-3-Ig)
FH

Daniela (Verified Customer) (06-03-2019)

In the figure, immunoblot of total cell lysate of HeLa cells transfected (left lane) with recombinant DNA expressing for a protein with the DDDDK-tag at its C-terminus. In the right lane, total cell lysate of HeLa moc transfection. Per each lane, 30ug of protein were loaded.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
DYKDDDDK tag Antibody Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture, validation (1:1000 dilution) in HeLa cells (Cat no:66008-3-Ig)