DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Publications

41

CloneNo.

2B3C4

Reviews (4)

Cat no : 66008-3-Ig

Synonyms

DDDDK tag, DYKDDDDK

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Validation Data Gallery

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  WB analysis of HEK-293 using 66008-3-Ig

  HEK-293 and DDDDK-DPM1 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-3-Ig (DDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Transfected HEK-293 using 66008-3-Ig

  IP result of anti-DDDDK tag (IP:66008-3-Ig, 5ug; Detection:66008-3-Ig 1:1000) with Transfected HEK-293 cells lysate 1600 ug.

• 

  IF Staining of Transfected HEK-293 using 66008-3-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66008-3-Ig (DDDDK tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Transfected HEK-293 cells
• Positive IP detected in: Transfected HEK-293 cells
• Positive IF detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Product Information

66008-3-Ig targets DYKDDDDK tag in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DYKDDDDK tag
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publications

Species	Application	Title
	IF	Fish Shellfish Immunol; Identification and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in common Chinese cuttlefish Sepiella japonica.; Authors - Zan Pang
	WB,IP	J Neurochem; Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.; Authors - Robert J Andrew
		Cell Death Dis; SPOP suppresses pancreatic cancer progression by promoting the degradation of NANOG.; Authors - Peng Tan
	WB	Mol Genet Genomic Med; A novel homozygous nonsense ZP1 variant causes human female infertility associated with empty follicle syndrome (EFS).; Authors - Qianhua Xu
		Cell Death Dis; FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation.; Authors - Xiao Liu
		Vet Parasitol; Recombinant invasive Lactobacillus plantarum expressing the Eimeria tenella fusion gene TA4 and AMA1 induces protection against coccidiosis in chickens.; Authors - Yang Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This is an excellent antibody for both IF and IB.

• Applications: Western Blot,Immunofluorescence
• Primary Antibody Dilution: 1:200 (IF)~2,000 (IB)
• Cell Tissue Type: HeLa and HEK293 cells

FH

Chun (Verified Customer) (12-05-2019)

Excellent antibody

• Applications: Western Blot,Immunohistochemistry,Immunofluorescence,
• Primary Antibody Dilution: 1:200 ~1: 2,000
• Cell Tissue Type: HEK293 and HeLa cells

FH

David (Verified Customer) (08-07-2019)

Bacterial Expression system with Flag tag. This seems to be very similar to the other mouse flag tag that we bought before. This works great and we got good results.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Bacterial

DYKDDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacterial (Cat no:66008-3-Ig)

FH

Daniela (Verified Customer) (06-03-2019)

In the figure, immunoblot of total cell lysate of HeLa cells transfected (left lane) with recombinant DNA expressing for a protein with the DDDDK-tag at its C-terminus. In the right lane, total cell lysate of HeLa moc transfection. Per each lane, 30ug of protein were loaded.

• Applications: Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

DYKDDDDK tag Antibody Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture, validation (1:1000 dilution) in HeLa cells (Cat no:66008-3-Ig)