DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Monoclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (1)
Applications
WB, RIP, IP, IHC, IF, CoIP
Conjugate
Unconjugated
CloneNo.
2B3C4
Cat no : 66008-3-Ig
Synonyms
"DYKDDDDK tag Antibodies" Comparison
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Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 48 publications below |
| IHC | See 1 publications below |
| IF | See 9 publications below |
| IP | See 9 publications below |
| CoIP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
66008-3-Ig targets DYKDDDDK tag in WB, RIP, IP, IHC, IF, CoIP applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | DYKDDDDK tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYKDDDDK tag antibody 66008-3-Ig | Download protocol |
| IF protocol for DYKDDDDK tag antibody 66008-3-Ig | Download protocol |
| IP protocol for DYKDDDDK tag antibody 66008-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Fish Shellfish Immunol Identification and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in common Chinese cuttlefish Sepiella japonica. | ||
J Neurochem Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms. | ||
Cell Death Dis SPOP suppresses pancreatic cancer progression by promoting the degradation of NANOG. | ||
Mol Genet Genomic Med A novel homozygous nonsense ZP1 variant causes human female infertility associated with empty follicle syndrome (EFS). | ||
Cell Death Dis FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation. | ||
Cell Death Dis PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christine (Verified Customer) (11-10-2021) | Very clean detection of overexpressed FLAG tagged protein in HEK293T cells.
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FH Jinwei (Verified Customer) (09-20-2021) | DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-3-Ig) works fine.
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FH Chun (Verified Customer) (09-07-2020) | This is an excellent antibody for both IF and IB.
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FH Chun (Verified Customer) (12-05-2019) | Excellent antibody
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FH David (Verified Customer) (08-07-2019) | Bacterial Expression system with Flag tag. This seems to be very similar to the other mouse flag tag that we bought before. This works great and we got good results.
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FH Daniela (Verified Customer) (06-03-2019) | In the figure, immunoblot of total cell lysate of HeLa cells transfected (left lane) with recombinant DNA expressing for a protein with the DDDDK-tag at its C-terminus. In the right lane, total cell lysate of HeLa moc transfection. Per each lane, 30ug of protein were loaded.
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