|Positive WB detected in||HEK-293 cells, human brain tissue, HepG2 cells, Neuro-2a cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue, C6 cells, Jurkat cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human testis tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:300|
|Sample-dependent, check data in validation data gallery|
13057-2-AP targets G3BP1 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||G3BP1 fusion protein Ag3728|
|Full Name||GTPase activating protein (SH3 domain) binding protein 1|
|Calculated molecular weight||466 aa, 52 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC006997|
|Gene ID (NCBI)||10146|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
|Product Specific Protocols|
|WB protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|IHC protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|IF protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|IP protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|FC protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Hum Mol Genet
Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.
RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.
Missing in Action: Dysfunctional RNA Metabolism in Oligodendroglial Cells as a Contributor to Neurodegenerative Diseases?
ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.
Am J Physiol Renal Physiol
Stress granules are formed in renal proximal tubular cells during metabolic stress and ischemic injury for cell survival.
J Biol Chem
The RNA-binding protein FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for GRIA2 mRNA processing.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Patryk (Verified Customer) (03-18-2021)
I used the antibody for immunofluorescence imaging to label stress granules induced by treating the cells with with 50µM sodium arsenite. Used the antibody at a dilution 1:100 overnight at 4°C. Worked perfectly well, strong and specific signal. I am very satisfied of this antibody and strongly recommend if for immunofluorescence.
Kun (Verified Customer) (03-23-2020)
Very specific and sensitive
Biao (Verified Customer) (03-11-2020)
This antibody is very specific and good quality.
Joshua (Verified Customer) (12-28-2019)
PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules.
Yuan (Verified Customer) (11-02-2019)
Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining.
Zeinab (Verified Customer) (08-19-2019)
It worked great
Erica (Verified Customer) (05-15-2019)
Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules.
Karthik (Verified Customer) (04-24-2019)
Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes.Cells permeabilized with 0.2% triton for 10 minutes
Tian (Verified Customer) (01-23-2019)
I used it for ICC and it worked Great.