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G3BP1 Polyclonal antibody
G3BP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 13057-2-AP
Validation Data Gallery
|Positive WB detected in||C6 cells, HEK-293 cells, human brain tissue, Neuro-2a cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||sodium arsenite treated HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
13057-2-AP targets G3BP1 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, rat, chicken, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||G3BP1 fusion protein Ag3728|
|Full Name||GTPase activating protein (SH3 domain) binding protein 1|
|Calculated molecular weight||466 aa, 52 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC006997|
|Gene ID (NCBI)||10146|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
|Product Specific Protocols|
|WB protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|IHC protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|IF protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|IP protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|FC protocol for G3BP1 antibody 13057-2-AP||Download protocol|
|Click here to view our Standard Protocols|
m6A enhances the phase separation potential of mRNA.
Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.
ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids.
RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether.
Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation.
A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (10-05-2023)
Good and strong signal.
Tatyana (Verified Customer) (01-21-2023)
Used for IP and WB of GFP-tagged overexpressed human G3BP1 (HEK293 cells). Lysates were subjected to IP using GFP-Trap beads. WB was done using semi-dry transfer. Antibody was used in 4% milk in TBST at 1:1,000 dilution. It could be reused up to 3 times. As the image shows, the antibody can successfully detect both endogenous and OE protein.
Tobias (Verified Customer) (10-25-2022)
Peter (Verified Customer) (10-24-2022)
Best G3BP1 antibody I've used for Western, IF and IP
Patryk (Verified Customer) (03-18-2021)
I used the antibody for immunofluorescence imaging to label stress granules induced by treating the cells with with 50µM sodium arsenite. Used the antibody at a dilution 1:100 overnight at 4°C. Worked perfectly well, strong and specific signal. I am very satisfied of this antibody and strongly recommend if for immunofluorescence.
Kun (Verified Customer) (03-23-2020)
Very specific and sensitive
Biao (Verified Customer) (03-11-2020)
This antibody is very specific and good quality.
Joshua (Verified Customer) (12-28-2019)
PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules.
Yuan (Verified Customer) (11-02-2019)
Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining.
Zeinab (Verified Customer) (08-19-2019)
It worked great
Erica (Verified Customer) (05-15-2019)
Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules.
Karthik (Verified Customer) (04-24-2019)
Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes.Cells permeabilized with 0.2% triton for 10 minutes
Tian (Verified Customer) (01-23-2019)
I used it for ICC and it worked Great.