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  • KD/KO Validated

G3BP1 Polyclonal antibody

G3BP1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13057-2-AP

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, HepG2 cells, Neuro-2a cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue, C6 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:300
Sample-dependent, check data in validation data gallery

Product Information

13057-2-AP targets G3BP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G3BP1 fusion protein Ag3728
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC006997
Gene symbol G3BP1
Gene ID (NCBI) 10146
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
WB protocol for G3BP1 antibody 13057-2-APDownload protocol
IHC protocol for G3BP1 antibody 13057-2-APDownload protocol
IF protocol for G3BP1 antibody 13057-2-APDownload protocol
IP protocol for G3BP1 antibody 13057-2-APDownload protocol
FC protocol for G3BP1 antibody 13057-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Hum Mol Genet

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.

Authors - Desiree M Baron
humanIF

Neuron

RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.

Authors - Jacob R Mann
IF

Neurochem Res

Missing in Action: Dysfunctional RNA Metabolism in Oligodendroglial Cells as a Contributor to Neurodegenerative Diseases?

Authors - Peter Hoch-Kraft
humanWB

Mol Cell

ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.

Authors - Bo Wang
mouseWB

Am J Physiol Renal Physiol

Stress granules are formed in renal proximal tubular cells during metabolic stress and ischemic injury for cell survival.

Authors - Shixuan Wang
  • KD Validated
mouseIF

J Biol Chem

The RNA-binding protein FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for GRIA2 mRNA processing.

Authors - Maeve Tischbein

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Patryk (Verified Customer) (03-18-2021)

I used the antibody for immunofluorescence imaging to label stress granules induced by treating the cells with with 50µM sodium arsenite. Used the antibody at a dilution 1:100 overnight at 4°C. Worked perfectly well, strong and specific signal. I am very satisfied of this antibody and strongly recommend if for immunofluorescence.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HCT116 cells
G3BP1 Antibody Immunofluorescence validation (1:100 dilution) in HCT116 cells (Cat no:13057-2-AP)
FH

Kun (Verified Customer) (03-23-2020)

Very specific and sensitive

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Biao (Verified Customer) (03-11-2020)

This antibody is very specific and good quality.

  • Applications: Western Blot,
  • Cell Tissue Type: hct-116
FH

Joshua (Verified Customer) (12-28-2019)

PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: human pancreatic adenocarcinoma cells
G3BP1 Antibody Immunofluorescence, validation (1:500 dilution) in human pancreatic adenocarcinoma cells (Cat no:13057-2-AP)
FH

Yuan (Verified Customer) (11-02-2019)

Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Hela
G3BP1 Antibody Immunofluorescence, validation (1:250 dilution) in Hela (Cat no:13057-2-AP)
FH

Zeinab (Verified Customer) (08-19-2019)

It worked great

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:400
FH

Erica (Verified Customer) (05-15-2019)

Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Cell Tissue Type: HeLa; MEF
FH

Karthik (Verified Customer) (04-24-2019)

Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes.Cells permeabilized with 0.2% triton for 10 minutes

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1: 500 overnight at 4 degrees
  • Cell Tissue Type: Primary motor neurons
G3BP1 Antibody Immunofluorescence, validation (1: 500 overnight at 4 degrees dilution) in Primary motor neurons (Cat no:13057-2-AP)
FH

Tian (Verified Customer) (01-23-2019)

I used it for ICC and it worked Great.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1;100
  • Cell Tissue Type: NIH3T3