Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HepG2 cells, MCF-7 cells, T47D cells, HeLa cells, Jurkat cells, Neuro-2a cells, C2C12 cells, C6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, mouse liver tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16276-1-AP targets G3BP2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9355 Product name: Recombinant human G3BP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 97-449 aa of BC011731 Sequence: LLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRGPPPRGGMAQKLGSGRGTGQMEGRFTGQRR Predict reactive species |
| Full Name | GTPase activating protein (SH3 domain) binding protein 2 |
| Calculated Molecular Weight | 482aa,54 kDa; 449aa,51 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC011731 |
| Gene Symbol | G3BP2 |
| Gene ID (NCBI) | 9908 |
| RRID | AB_2878237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UN86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for G3BP2 antibody 16276-1-AP | Download protocol |
| IHC protocol for G3BP2 antibody 16276-1-AP | Download protocol |
| IP protocol for G3BP2 antibody 16276-1-AP | Download protocol |
| WB protocol for G3BP2 antibody 16276-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The G3BP2 polyclonal antibody (Cat# 16276-1-AP) has accumulated 51 citations across high-impact journals including Nature, Cell, Nature Cell Biology, Nature Communications, Molecular Cell, Nucleic Acids Research, Advanced Science, Oncogene, EMBO Journal, Cell Reports, and others. Associated research fields encompass stress granule assembly and dynamics, liquid-liquid phase separation, cancer initiation and progression, neuroinflammation, viral replication, ferroptosis regulation, and RNA metabolism.
| Species | Application | Title |
|---|---|---|
Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. | ||
Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules | ||
Mol Cancer Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.
| ||
Cancer Commun (Lond) BAALC-AS1/G3BP2/c-Myc feedback loop promotes cell proliferation in esophageal squamous cell carcinoma. | ||
Reviews
What customers say
Both reviewers gave 5-star ratings. One user reported clean bands in Western blot and good signal in immunofluorescence, noting the antibody is specific. The other performed Western blot on H69 cells at a 1:1000 dilution, using standard SDS-PAGE and ECL detection, and shared an image confirming clear results. Overall, users find this antibody reliable and specific for both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rhys (Verified Customer) (08-12-2026) | Clean band for Western blot and good signal for IF. Specific
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Karine (Verified Customer) (09-22-2022) | Proteins were extracted from H69 cells using Laemmli lysis buffer (12.5mM Na2HPO4, 15% glycerol, 3% sodium dodecyl sulfate [SDS]). The protein concentration was measured with the DC Protein Assay (BIO-RAD) and 30μg of total proteins were loaded onto 12% SDS- polyacrylamide gels for electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After 1h of blocking with 5% bovine serum albumin prepared in Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer, the blots were incubated overnight at 4°C with the indicated antibody (dilution 1/1000). After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:5,000, Promega), protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme).
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