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  • KD/KO Validated

G3BP2 Polyclonal antibody

G3BP2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16276-1-AP

Synonyms

G3BP 2, G3BP2, KIAA0660



Tested Applications

Positive WB detected inA549 cells, T47D cells, MCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, Neuro-2a cells
Positive IP detected inHeLa cells
Positive IF detected insodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

16276-1-AP targets G3BP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G3BP2 fusion protein Ag9355
Full Name GTPase activating protein (SH3 domain) binding protein 2
Calculated molecular weight482aa,54 kDa; 449aa,51 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC011731
Gene symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Protocols

Product Specific Protocols
WB protocol for G3BP2 antibody 16276-1-APDownload protocol
IF protocol for G3BP2 antibody 16276-1-APDownload protocol
IP protocol for G3BP2 antibody 16276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
humanWB,IF,IP

Oncogene

RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2.

Authors - Yaqi Chen
  • KD Validated
humanWB,IHC

Mol Cancer

Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.

Authors - Hongwei Liu
  • KD Validated
humanWB

Cancer Commun (Lond)

BAALC-AS1/G3BP2/c-Myc feedback loop promotes cell proliferation in esophageal squamous cell carcinoma.

Authors - Hongyue Zhang
humanIP

J Virol

SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication.

Authors - Hainan Liu
humanWB,IF

Front Cell Infect Microbiol

Identification of a non-canonical G3BP-binding sequence in a Mayaro virus nsP3 hypervariable domain

Authors - Aymeric Neyret
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Karine (Verified Customer) (09-22-2022)

Proteins were extracted from H69 cells using Laemmli lysis buffer (12.5mM Na2HPO4, 15% glycerol, 3% sodium dodecyl sulfate [SDS]). The protein concentration was measured with the DC Protein Assay (BIO-RAD) and 30μg of total proteins were loaded onto 12% SDS- polyacrylamide gels for electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After 1h of blocking with 5% bovine serum albumin prepared in Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer, the blots were incubated overnight at 4°C with the indicated antibody (dilution 1/1000). After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:5,000, Promega), protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: H69 cells
G3BP2 Antibody Western Blot validation (1/1000 dilution) in H69 cells (Cat no:16276-1-AP)