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  • KD/KO Validated

G3BP2 Polyclonal antibody

G3BP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16276-1-AP

Synonyms

G3BP 2, G3BP2, KIAA0660



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, Jurkat cells, T47D cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected insodium arsenite treated HeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16276-1-AP is G3BP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G3BP2 fusion protein Ag9355
Full Name GTPase activating protein (SH3 domain) binding protein 2
Calculated molecular weight482aa,54 kDa; 449aa,51 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC011731
Gene symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Protocols

Product Specific Protocols
WB protocol for G3BP2 antibody 16276-1-APDownload protocol
IHC protocol for G3BP2 antibody 16276-1-APDownload protocol
IF protocol for G3BP2 antibody 16276-1-APDownload protocol
IP protocol for G3BP2 antibody 16276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

J Virol

SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication.

Authors - Hainan Liu
humanWB

Cancer Commun (Lond)

BAALC-AS1/G3BP2/c-Myc feedback loop promotes cell proliferation in esophageal squamous cell carcinoma.

Authors - Hongyue Zhang
humanWB,IHC

Mol Cancer

Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.

Authors - Hongwei Liu
  • KD Validated
humanWB,IF,IP

Oncogene

RIOK1 mediates p53 degradation and radioresistance in colorectal cancer through phosphorylation of G3BP2.

Authors - Yaqi Chen
  • KD Validated
human

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang