Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, HCT 116 cells, MCF-7 cells, U2OS cells, NIH/3T3 cells, PC-12 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11858-1-AP targets Galectin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2432 Product name: Recombinant human Galectin 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC020675 Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD Predict reactive species |
| Full Name | lectin, galactoside-binding, soluble, 1 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 10-15 kDa |
| GenBank Accession Number | BC020675 |
| Gene Symbol | Galectin-1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_2234673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
| IHC protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
| WB protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Galectin-1 Polyclonal antibody (Cat# 11858-1-AP) has 33 citations across journals including Nature Communications, Cell Reports, Developmental Cell, Cancer Letters, Cell & Molecular Immunology, International Immunopharmacology, and FEBS Journal. Research fields span oncology (glioma, renal, gastric, lung, ovarian, breast, melanoma, AML), fibrosis (pulmonary, hepatic), immune regulation, wound healing, neurology, bone biology, atherosclerosis, and infectious disease.
| Species | Application | Title |
|---|---|---|
Adv Mater A Hierarchical Plug-and-Supply Flap (PASF) for Instant Perfusion and Oxygen Delivery in Large Soft Tissue Repair. | ||
Cell Mol Immunol Oxidized galectin-1 in SLE fails to bind the inhibitory receptor VSTM1 and increases reactive oxygen species levels in neutrophils | ||
Bioact Mater Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment | ||
Nat Commun Galectin-3-integrin α5β1 phase separation disrupted by advanced glycation end-products impairs diabetic wound healing in rodents. | ||
J Exp Clin Cancer Res Nuclear FAM289-Galectin-1 interaction controls FAM289-mediated tumor promotion in malignant glioma. | ||
Cancer Lett Cancer-associated fibroblasts promote immune evasion in pancreatic cancer via miR-181b-5p/STING/LGALS1 pathway. |
Reviews
What customers say
The single review for 11858-1-AP is highly positive, rated 5 stars. The reviewer reports the antibody works well for immunofluorescence on human tissue and cell lines. They also note successfully validating the antibody using an overexpressing cell line. Overall, the feedback highlights reliable performance in IF applications with human samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jennifer (Verified Customer) (08-19-2026) | Works well for IF on human tissue. We have also successfully validated this antibody using an overexpressing cell line.
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