Galectin-1 Polyclonal antibody

• 

  WB analysis of HepG2 using 11858-1-AP

  WB result of Galectin-1 antibody (11858-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells.

• 

  WB analysis using 11858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 11858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Galectin-1 antibody (11858-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

Galectin-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11858-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GAL1, Gal 1, Beta-galactoside-binding lectin L-14-I, 14 kDa lectin, 14 kDa laminin-binding protein

---

Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, MCF-7 cells, U2OS cells
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

11858-1-AP targets Galectin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galectin-1 fusion protein Ag2432
• Full Name: lectin, galactoside-binding, soluble, 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC020675
• Gene Symbol: Galectin-1
• Gene ID (NCBI): 3956
• RRID: AB_2234673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09382
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Publications

Species	Application	Title
human,mouse	WB,IF	Dev Cell; Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption; Authors - Mobai Li
human	IF	Front Med; Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis; Authors - Weiwei Xian
human	WB	Cell Rep; Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.; Authors - Krishna S Tummala
human	WB	Mol Ther Nucleic Acids; Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.; Authors - Chen Yang
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
mouse	IHC	Biomed Pharmacother; Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction.; Authors - Yoojin Seo