Galectin-1 Polyclonal antibody
Galectin-1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11858-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, human heart tissue, human liver tissue, A549 cells, L02 cells, NIH/3T3 cells, PC-12 cells, HCT 116 cells, MCF-7 cells, U2OS cells |
Positive IHC detected in | human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 9 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
Product Information
11858-1-AP targets Galectin-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Galectin-1 fusion protein Ag2432 |
Full Name | lectin, galactoside-binding, soluble, 1 |
Calculated molecular weight | 15 kDa |
Observed molecular weight | 14 kDa |
GenBank accession number | BC020675 |
Gene symbol | LGALS1 |
Gene ID (NCBI) | 3956 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Protocols
Product Specific Protocols | |
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WB protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
IHC protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
IF protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells. | ||
Mol Ther Nucleic Acids Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. | ||
Biomed Pharmacother Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction. | ||
Int J Oncol Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer | ||
Front Med (Lausanne) An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury. |