Validation Data Gallery
|Positive WB detected in
|A549 cells, HepG2 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, MCF-7 cells, U2OS cells
|Positive IHC detected in
|human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:12000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11858-1-AP targets Galectin-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Galectin-1 fusion protein Ag2432
|lectin, galactoside-binding, soluble, 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.
Mol Ther Nucleic Acids
Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.
Prognostic Value and Biological Function of Galectins in Malignant Glioma.
Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction.
Int J Oncol
Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer
Front Med (Lausanne)
An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.