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Galectin-1 Polyclonal antibody

Galectin-1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11858-1-AP

Synonyms

14 kDa laminin binding protein, 14 kDa lectin, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11858-1-AP targets Galectin-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432
Full Name lectin, galactoside-binding, soluble, 1
Calculated molecular weight 15 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC020675
Gene symbol LGALS1
Gene ID (NCBI) 3956
RRIDAB_2234673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Protocols

Product Specific Protocols
WB protocol for Galectin-1 antibody 11858-1-APDownload protocol
IHC protocol for Galectin-1 antibody 11858-1-APDownload protocol
IF protocol for Galectin-1 antibody 11858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Authors - Krishna S Tummala
humanWB

Mol Ther Nucleic Acids

Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.

Authors - Chen Yang
humanWB,IHC

Front Oncol

Prognostic Value and Biological Function of Galectins in Malignant Glioma.

Authors - Hongtao Zhu
mouseIHC

Biomed Pharmacother

Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction.

Authors - Yoojin Seo
humanIHC

Int J Oncol

Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

Authors - Qi Zhang
mouseIHC

Front Med (Lausanne)

An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.

Authors - Songyan Gao