CoraLite®594-conjugated Galectin-1 Polyclonal antibody

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  IF Staining of rat colon using CL594-11858

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat colon using CL594-11858

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using CL594-11858

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Galectin-1 antibody (CL594-11858) at dilution of 1:200.

• Featured Product
• KD/KO Validated

Galectin-1 Polyclonal Antibody for IF/ICC, IF-P, ELISA

Cat No. CL594-11858

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, ELISA

Synonyms

Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1, Galaptin

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Tested Applications

• Positive IF-P detected in: rat colon tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11858 targets Galectin-1 in IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galectin-1 fusion protein Ag2432
• Full Name: lectin, galactoside-binding, soluble, 1
• Calculated Molecular Weight: 15 kDa
• GenBank Accession Number: BC020675
• Gene Symbol: Galectin-1
• Gene ID (NCBI): 3956
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09382
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).