Validation Data Gallery
|Positive WB detected in||mouse brain tissue, U-251 cells|
|Positive IHC detected in||human brain tissue, rat brain tissue, mouse brain tissue, human gliomas tissue, Alzheimer mouse|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||rat brain tissue, mouse brain tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16825-1-AP targets GFAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||goat, hamster, human, mouse, pig, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GFAP fusion protein Ag10423|
|Full Name||glial fibrillary acidic protein|
|Calculated molecular weight||432 aa, 50 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC013596|
|Gene ID (NCBI)||2670|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FunctionGFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.
Tissue specificityGFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).
Involvement in disease
- Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
- Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.
IsoformsAstrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.
Post-translational modificationsIntermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).
Cellular localizationGFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (09-30-2021)
Immunofluorescence works well on U251-MG human astrocytoma cell line
Azita (Verified Customer) (05-31-2021)
The human primary cortical cells (DIV28) were subjected to ICC using GFAP antibody (at 1/500 dilution) overnight at 4°C.
Diane (Verified Customer) (01-03-2020)
I have used this antibody successfully for formalin-fixed paraffin-embedded brain tissues in humans, mouse and rat. I am impressed that there is no non-specific background staining regardless of species. We have used the antibody in a double-staining technique and were able to achieve crisp diagnostic staining for images. The astrocytes were stained using alkaline phosphatase Permanent Red. Antigen retrieval was performed using proteinase K.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good immuno-fluorescence and western blotting analysis using mouse brain tissues.
Ryan (Verified Customer) (02-14-2018)
NaCit antigen retrieval ph=6