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GFAP Polyclonal antibody

GFAP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (5)





Cat no : 16825-1-AP



Tested Applications

Positive WB detected inmouse brain tissue, U-251 cells, rat brain tissue
Positive IHC detected inmouse brain tissue, Alzheimer mouse
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, mouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16825-1-AP targets GFAP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, zebrafish, hamster, pig, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFAP fusion protein Ag10423
Full Name glial fibrillary acidic protein
Calculated Molecular Weight 432 aa, 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC013596
Gene Symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.
Tissue specificity
GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).
Involvement in disease
  • Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
  • Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.
Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.
Post-translational modifications
Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).
Cellular localization
GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.


Product Specific Protocols
WB protocol for GFAP antibody 16825-1-APDownload protocol
IHC protocol for GFAP antibody 16825-1-APDownload protocol
IF protocol for GFAP antibody 16825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Vikas (Verified Customer) (07-11-2024)

Used for IF, Worked very well, Highly recommended.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Brain tissue

manohar (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

Badrieh (Verified Customer) (08-02-2022)

this Antibody worked really great with different Recombinant GFAP proteins in ELISA.

  • Applications: Other

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence works well on U251-MG human astrocytoma cell line

  • Applications: Immunohistochemistry, Immunofluorescence

Azita (Verified Customer) (05-31-2021)

The human primary cortical cells (DIV28) were subjected to ICC using GFAP antibody (at 1/500 dilution) overnight at 4°C.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: Human primary cortical cells

Diane (Verified Customer) (01-03-2020)

I have used this antibody successfully for formalin-fixed paraffin-embedded brain tissues in humans, mouse and rat. I am impressed that there is no non-specific background staining regardless of species. We have used the antibody in a double-staining technique and were able to achieve crisp diagnostic staining for images. The astrocytes were stained using alkaline phosphatase Permanent Red. Antigen retrieval was performed using proteinase K.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: gliotic brain tissue - human
GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in gliotic brain tissue - human (Cat no:16825-1-AP)

Tanusree (Verified Customer) (12-03-2019)

This antibody works good immuno-fluorescence and western blotting analysis using mouse brain tissues.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph=6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Zebrafish retina 5dpf
GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in Zebrafish retina 5dpf (Cat no:16825-1-AP)