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GFAP Antibody 70 Publications

Rabbit Polyclonal| Catalog number: 16825-1-AP

Featured Product KD/KO validated

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Con: 65 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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GFAP Antibody IF mouse brain tissue 16825-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 16825-1-AP (GFAP antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

GFAP Antibody IF mouse brain tissue 16825-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse brain tissue using 16825-1-AP(GFAP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

GFAP Antibody WB U-251 cells 16825-1-AP

WB result of GFAP antibody (16825-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells

GFAP Antibody IHC Alzheimer mouse 16825-1-AP

Immunohistochemical analysis of paraffin-embedded Hippocampus tissue slide from Alzheimer Mouse Model using 16825-1-AP (GFAP antibody) at dilution of 1:50000 (under 10x lens). Data from NeuroScience Associates, Inc.

GFAP Antibody IHC human brain tissue 16825-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16825-1-AP( GFAP Antibody) at dilution of 1:2000 (under 10x lens).

GFAP Antibody IF mouse brain tissue 16825-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 16825-1-AP (GFAP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

GFAP Antibody IHC human brain tissue 16825-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16825-1-AP( GFAP Antibody) at dilution of 1:2000 (under 40x lens).

GFAP Antibody IHC rat brain tissue 16825-1-AP

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:200 (under 10x lens).

GFAP Antibody WB mouse brain tissue 16825-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 16825-1-AP (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat, hamster, hamsters, swine
Positive WB detected in:
mouse brain tissue, U-251 cells
Positive IHC detected in:
human brain tissue, mouse brain tissue, rat brain tissue, Alzheimer mouse, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:5000-1:50000
IHC : 1:50-1:2000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GFAP fusion protein Ag10423
Full name:
glial fibrillary acidic protein
Calculated molecular weight:
432aa,50 kDa
Observed molecular weight:
45-50 kDa
GenBank accession number:
BC013596
Gene ID (NCBI):
2670
Gene symbol
GFAP
Synonyms
GFAP

Background

Function
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.
Tissue specificity
GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).
Involvement in disease
  • Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
  • Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer’s and Parkinson’s disease), and neurotrauma.
Isoforms
Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.
Post-translational modifications
Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).
Cellular localization
GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

GFAP Rabbit Polyclonal antibody

Ryan (Verified Customer), 02-15-2018
Applications
Immunohistochemistry,
Primary Antibody Dilution
1:200
Secondary Antibody Dilution
1:1000
GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in Zebrafish retina 5dpf (Cat no:16825-1-AP)

GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in Zebrafish retina 5dpf (Cat no:16825-1-AP)

Cell/Tissue Type
Zebrafish retina 5dpf
Lot Number
00023078
NaCit antigen retrieval ph=6

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