Beta Galactosidase Polyclonal antibody
Beta Galactosidase Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15518-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells |
Positive IHC detected in | human gliomas tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 8 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
Product Information
15518-1-AP targets Beta Galactosidase in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Beta Galactosidase fusion protein Ag7792 |
Full Name | galactosidase, beta 1 |
Calculated molecular weight | 76 kDa |
Observed molecular weight | 67 kDa |
GenBank accession number | BC007493 |
Gene symbol | GLB1 |
Gene ID (NCBI) | 2720 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
What is the molecular weight of β-galactosidase (GLB1)?
The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lower
molecular weight values.
What is the function of GLB1?
The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzing the hydrolysis of
lactose to monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymeric D-galactosyl
residues (PMID: 23526073, doi: 10.1088/1757-899x/263/2/022046).
How is GLB1 used in our food?
GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturing
of lactose-hydrolyzed products (PMID: 28500401).
How is GLB1 part of a positive feedback loop?
GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon and
binds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).
What organisms express GLB1?
GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals.
Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1
prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values
(PMID: 28500401).
How is GLB1 utilized in transgenic animals?
Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. The lacZ gene of E. coli
has been used to identify cis-acting DNA elements that play a key role for the regulation of gene expression
in mice. The lacZ gene can be incorporated into an endogenous gene, and it is frequently a used to indicate
the success of Cre-loxP recombination events, which allows temporal control of lacZ expression. Mice a
part of the Rosa26R line that also express Cre ubiquitously express Cre-inducible lacZ (PMID: 26394634).
Protocols
Product Specific Protocols | |
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WB protocol for Beta Galactosidase antibody 15518-1-AP | Download protocol |
IHC protocol for Beta Galactosidase antibody 15518-1-AP | Download protocol |
IF protocol for Beta Galactosidase antibody 15518-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence. | ||
Cell Rep Senescence Induced by BMI1 Inhibition Is a Therapeutic Vulnerability in H3K27M-Mutant DIPG. | ||
Pain Res Manag OPN Deficiency Increases the Severity of Osteoarthritis Associated with Aberrant Chondrocyte Senescence and Apoptosis and Upregulates the Expression of Osteoarthritis-Associated Genes. | ||
Biosci Rep Active PKG II inhibited the growth and migration of ovarian cancer cells through blocking Raf/ MEK and PI3K/Akt signaling pathways. | ||