Beta Galactosidase Polyclonal antibody

Beta Galactosidase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15518-1-AP

Synonyms

Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase



Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15518-1-AP targets Beta Galactosidase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Galactosidase fusion protein Ag7792
Full Name galactosidase, beta 1
Calculated molecular weight 76 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC007493
Gene symbol GLB1
Gene ID (NCBI) 2720
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the molecular weight of β-galactosidase (GLB1)?

The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lower 

molecular weight values.


What is the function of GLB1?

The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzing the hydrolysis of 

lactose to monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymeric D-galactosyl 

residues (PMID: 23526073, doi: 10.1088/1757-899x/263/2/022046).


How is GLB1 used in our food?

GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturing 

of lactose-hydrolyzed products (PMID: 28500401).


How is GLB1 part of a positive feedback loop?

GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon and 

binds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).


What organisms express GLB1?

GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals. 

Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1 

prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values

(PMID: 28500401).


How is GLB1 utilized in transgenic animals?

Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. The lacZ gene of E. coli 

has been used to identify cis-acting DNA elements that play a key role for the regulation of gene expression 

in mice. The lacZ gene can be incorporated into an endogenous gene, and it is frequently a used to indicate 

the success of Cre-loxP recombination events, which allows temporal control of lacZ expression. Mice a 

part of the Rosa26R line that also express Cre ubiquitously express Cre-inducible lacZ (PMID: 26394634).


Protocols

Product Specific Protocols
WB protocol for Beta Galactosidase antibody 15518-1-APDownload protocol
IHC protocol for Beta Galactosidase antibody 15518-1-APDownload protocol
IF protocol for Beta Galactosidase antibody 15518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cachexia Sarcopenia Muscle

Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.

Authors - Guo-Kai Shang
humanIHC

Theranostics

Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.

Authors - Gengyi Zou
mouseWB,IF

Cancer Lett

Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation.

Authors - Long Shen
mouseWB

Arterioscler Thromb Vasc Biol

β1-Integrin Is Essential for Vasoregulation and Smooth Muscle Survival In Vivo.

Authors - Turlo Kirsten A KA
humanIP

Cell Death Dis

Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.

Authors - Christos E Zois
ratWB

Aging (Albany NY)

Spermidine alleviates cardiac aging by improving mitochondrial biogenesis and function.

Authors - Junying Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lin (Verified Customer) (02-24-2022)

It is good for WB.

  • Applications: Western Blot
FH

Lin (Verified Customer) (06-04-2021)

It works for skin IHC.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: human skin