Beta galactosidase Polyclonal antibody

Beta galactosidase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 15518-1-AP

Synonyms

Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 15518-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Hela using 15518-1-AP

  Immunofluorescent analysis of Hela cells, using GLB1 antibody 15518-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IHC staining of human gliomas using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta galactosidase Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta galactosidase Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: human gliomas tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

15518-1-AP targets Beta galactosidase in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta galactosidase fusion protein Ag7792
• Full Name: galactosidase, beta 1
• Calculated molecular weight: 76 kDa
• Observed molecular weight: 67 kDa
• GenBank accession number: BC007493
• Gene symbol: GLB1
• Gene ID (NCBI): 2720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

What is the molecular weight of β-galactosidase (GLB1)?

The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lower 

molecular weight values.

What is the function of GLB1?

The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzing the hydrolysis of 

lactose to monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymeric D-galactosyl 

residues (PMID: 23526073, doi: 10.1088/1757-899x/263/2/022046).

How is GLB1 used in our food?

GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturing 

of lactose-hydrolyzed products (PMID: 28500401).

How is GLB1 part of a positive feedback loop?

GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon and 

binds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).

What organisms express GLB1?

GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals. 

Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1 

prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values

(PMID: 28500401).

How is GLB1 utilized in transgenic animals?

Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. The lacZ gene of E. coli 

has been used to identify cis-acting DNA elements that play a key role for the regulation of gene expression 

in mice. The lacZ gene can be incorporated into an endogenous gene, and it is frequently a used to indicate 

the success of Cre-loxP recombination events, which allows temporal control of lacZ expression. Mice a 

part of the Rosa26R line that also express Cre ubiquitously express Cre-inducible lacZ (PMID: 26394634).

Publications

Species	Application	Title
human	WB,IHC	Signal Transduct Target Ther; Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence.; Authors - Yingxi Li
human	IF	Aging Cell; Galactose-modified duocarmycin prodrugs as senolytics.; Authors - Ana Guerrero ; KD Validated
human	WB	Biosci Rep; Active PKG II inhibited the growth and migration of ovarian cancer cells through blocking Raf/ MEK and PI3K/Akt signaling pathways.; Authors - Yan Wu
mouse	WB	Atherosclerosis; PMID: 21396644; Authors - Werner Christian C
human	WB	Exp Gerontol; Unhealthy diet and ultrafine carbon black particles induce senescence and disease associated phenotypic changes.; Authors - Büchner Nicole N
human	IF	Stem Cell Res Ther; Extracellular vesicles from adipose-derived stem cells ameliorate ultraviolet B-induced skin photoaging by attenuating reactive oxygen species production and inflammation.; Authors - Peng Xu