Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | human prostate cancer tissue, human gliomas tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue, human prostate cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 26 publications below |
| IHC | See 14 publications below |
| IF | See 10 publications below |
| IP | See 1 publications below |
Product Information
15518-1-AP targets Beta Galactosidase in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Beta Galactosidase fusion protein Ag7792 Predict reactive species |
| Full Name | galactosidase, beta 1 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC007493 |
| Gene Symbol | GLB1/Beta-galactosidase |
| Gene ID (NCBI) | 2720 |
| RRID | AB_2263448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16278 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the molecular weight of β-galactosidase (GLB1)?
The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lower
molecular weight values.
What is the function of GLB1?
The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzing the hydrolysis of
lactose to monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymeric D-galactosyl
residues (PMID: 23526073, doi: 10.1088/1757-899x/263/2/022046).
How is GLB1 used in our food?
GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturing
of lactose-hydrolyzed products (PMID: 28500401).
How is GLB1 part of a positive feedback loop?
GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon and
binds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).
What organisms express GLB1?
GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals.
Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1
prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values
(PMID: 28500401).
How is GLB1 utilized in transgenic animals?
Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. The lacZ gene of E. coli
has been used to identify cis-acting DNA elements that play a key role for the regulation of gene expression
in mice. The lacZ gene can be incorporated into an endogenous gene, and it is frequently a used to indicate
the success of Cre-loxP recombination events, which allows temporal control of lacZ expression. Mice a
part of the Rosa26R line that also express Cre ubiquitously express Cre-inducible lacZ (PMID: 26394634).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Galactosidase antibody 15518-1-AP | Download protocol |
| IHC protocol for Beta Galactosidase antibody 15518-1-AP | Download protocol |
| IF protocol for Beta Galactosidase antibody 15518-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity | ||
Environ Int HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity | ||
J Cachexia Sarcopenia Muscle Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles. | ||
Theranostics Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. | ||
Cancer Lett Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Maelle (Verified Customer) (12-19-2024) | Functional
|
FH Brice-Emmanuel (Verified Customer) (12-18-2023) | Work well in WB
 |
FH Lin (Verified Customer) (02-24-2022) | It is good for WB.
|
FH Lin (Verified Customer) (06-04-2021) | It works for skin IHC.
|
