Beta Galactosidase Polyclonal antibody, PBS Only

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  WB analysis of SH-SY5Y using 15518-1-AP (same clone as 15518-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 15518-1-AP (same clone as 15518-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta galactosidase Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human prostate cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 15518-1-AP (same clone as 15518-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15518-1-AP (same clone as 15518-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

Beta Galactosidase Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 15518-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

GLB1, Acid beta-galactosidase, Beta-galactosidase, EBP, EC:3.2.1.23

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Product Information

15518-1-PBS targets Beta Galactosidase in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Galactosidase fusion protein Ag7792
• Full Name: galactosidase, beta 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC007493
• Gene Symbol: GLB1/Beta-galactosidase
• Gene ID (NCBI): 2720
• RRID: AB_2263448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16278
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

What is the molecular weight of β-galactosidase (GLB1)?

The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lower 

molecular weight values.

What is the function of GLB1?

The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzing the hydrolysis of 

lactose to monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymeric D-galactosyl 

residues (PMID: 23526073, doi: 10.1088/1757-899x/263/2/022046).

How is GLB1 used in our food?

GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturing 

of lactose-hydrolyzed products (PMID: 28500401).

How is GLB1 part of a positive feedback loop?

GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon and 

binds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).

What organisms express GLB1?

GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals. 

Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1 

prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values

(PMID: 28500401).

How is GLB1 utilized in transgenic animals?

Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. The lacZ gene of E. coli 

has been used to identify cis-acting DNA elements that play a key role for the regulation of gene expression 

in mice. The lacZ gene can be incorporated into an endogenous gene, and it is frequently a used to indicate 

the success of Cre-loxP recombination events, which allows temporal control of lacZ expression. Mice a 

part of the Rosa26R line that also express Cre ubiquitously express Cre-inducible lacZ (PMID: 26394634).