Beta Galactosidase Polyclonal antibody, PBS Only

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  WB analysis of SH-SY5Y using 15518-1-AP (same clone as 15518-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 15518-1-AP (same clone as 15518-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta galactosidase Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human prostate cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15518-1-AP (same clone as 15518-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

Beta Galactosidase Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 15518-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

GLB1, Acid beta-galactosidase, Beta-galactosidase, EBP, EC:3.2.1.23

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Product Information

15518-1-PBS targets Beta Galactosidase in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Galactosidase fusion protein Ag7792
• Full Name: galactosidase, beta 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC007493
• Gene Symbol: GLB1/Beta-galactosidase
• Gene ID (NCBI): 2720
• RRID: AB_2263448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16278
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.