Tested Applications
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, PC-12 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, K-562 cells |
| Positive IF/ICC detected in | HeLa cells, C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66662-1-Ig targets GOLGA2/GM130 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, duck |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species |
| Full Name | golgi autoantigen, golgin subfamily a, 2 |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC014188 |
| Gene Symbol | GM130 |
| Gene ID (NCBI) | 2801 |
| RRID | AB_2882017 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q08379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GOLGA2/GM130 antibody 66662-1-Ig | Download protocol |
| WB protocol for GOLGA2/GM130 antibody 66662-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GOLGA2/GM130 Monoclonal antibody (66662-1-Ig) has 41 total citations. It appears across high-impact journals including Nature Communications, Journal of Extracellular Vesicles, Molecular Cell, Cancer Communications, Advanced Science, Cell Reports, Cell Death & Disease, Journal of Nanobiotechnology, Molecular Metabolism, PLoS Pathogens, and Cell & Molecular Immunology. Research fields span extracellular vesicle/exosome biology, cancer (hepatocellular, breast, pancreatic, ovarian, prostate), Golgi apparatus and primary cilia cell biology, liver injury, cardiovascular disease, neurobiology, and immunology, applied primarily via Western blot and immunofluorescence across human, mouse, rat, and duck samples.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma | ||
Cell Mol Immunol FBXW7 is a multifaceted regulator of the innate immune response to DNA viruses | ||
J Extracell Vesicles Tumour-Derived Extracellular Vesicles Induce Diffuse Large B-Cell Lymphoma Progression Through Macrophage-Mediated MDSC Accumulation. | ||
J Extracell Vesicles Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. | ||
Nat Commun Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity | ||
