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GOLGA2/GM130 Monoclonal antibody

GOLGA2/GM130 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Rat and More (2)







Cat no : 66662-1-Ig


Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, K-562 cells, PC-12 cells
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

66662-1-Ig targets GOLGA2/GM130 in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC014188
Gene Symbol GOLGA2
Gene ID (NCBI) 2801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)


Product Specific Protocols
WB protocol for GOLGA2/GM130 antibody 66662-1-IgDownload protocol
IF protocol for GOLGA2/GM130 antibody 66662-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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