GNB3 Monoclonal antibody

GNB3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6E1

Cat no : 67497-1-Ig

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 2B6E1

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Validation Data Gallery

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  WB analysis using 67497-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67497-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 67497-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67497-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat retina using 67497-1-Ig

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67497-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: hTERT-RPE1 cells, HepG2 cells, L02 cells, pig brain tissue, rat retina tissue, Raji cells, Ramos cells, Daudi cells, RKO cells, Karpas-422 cells, IMR-32 cells, Rat brain cells, Mouse brain cells, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67497-1-Ig targets GNB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNB3 fusion protein Ag7050
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC002454
• Gene Symbol: GNB3
• Gene ID (NCBI): 2784
• RRID: AB_2882721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.