• Featured Product
  • KD/KO Validated

GOLPH3 Polyclonal antibody

GOLPH3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19112-1-AP

Synonyms

Coat protein GPP34, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, rat testis tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue, human placenta tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

19112-1-AP targets GOLPH3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLPH3 fusion protein Ag5443
Full Name golgi phosphoprotein 3 (coat-protein)
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC033725
Gene symbol GOLPH3
Gene ID (NCBI) 64083
RRIDAB_2113342
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

Protocols

Product Specific Protocols
WB protocol for GOLPH3 antibody 19112-1-APDownload protocol
IHC protocol for GOLPH3 antibody 19112-1-APDownload protocol
IF protocol for GOLPH3 antibody 19112-1-APDownload protocol
IP protocol for GOLPH3 antibody 19112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Theranostics

miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.

Authors - Qing Zhang
  • KD Validated
humanWB

Cancer Res

A Novel Micropeptide Encoded by Y-Linked LINC00278 Links Cigarette Smoking and AR Signaling in Male Esophageal Squamous Cell Carcinoma.

Authors - Siqi Wu
humanWB

Cell Death Dis

GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer

Authors - Jun-Wei Song
humanIHC

Front Oncol

FAP-a and GOLPH3 Are Hallmarks of DCIS Progression to Invasive Breast Cancer.

Authors - Li-Na Yu

Br J Cancer

GOLPH3 is a novel marker of poor prognosis and a potential therapeutic target in human renal cell carcinoma.

Authors - Y Xue
humanWB

J Exp Clin Cancer Res

STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.

Authors - Fan Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-17-2023)

fairly good for IF, but not as clear as the images in its booklet

  • Applications: Immunofluorescence