Validation Data Gallery
|Positive WB detected in
|HeLa cells, mouse lung tissue, rat testis tissue, mouse testis tissue
|Positive IP detected in
|mouse testis tissue
|Positive IHC detected in
|human colon cancer tissue, human placenta tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
19112-1-AP targets GOLPH3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|GOLPH3 fusion protein Ag5443
|golgi phosphoprotein 3 (coat-protein)
|Calculated molecular weight
|298 aa, 34 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).
|Product Specific Protocols
|WB protocol for GOLPH3 antibody 19112-1-AP
|IHC protocol for GOLPH3 antibody 19112-1-AP
|IF protocol for GOLPH3 antibody 19112-1-AP
|IP protocol for GOLPH3 antibody 19112-1-AP
|Click here to view our Standard Protocols
miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.
A Novel Micropeptide Encoded by Y-Linked LINC00278 Links Cigarette Smoking and AR Signaling in Male Esophageal Squamous Cell Carcinoma.
Cell Death Dis
GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer
FAP-a and GOLPH3 Are Hallmarks of DCIS Progression to Invasive Breast Cancer.
Br J Cancer
GOLPH3 is a novel marker of poor prognosis and a potential therapeutic target in human renal cell carcinoma.
J Exp Clin Cancer Res
STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (04-17-2023)
fairly good for IF, but not as clear as the images in its booklet