|Positive WB detected in||A549 cells, pig brain tissue, rat brain tissue, NCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
66627-1-Ig targets GORASP2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Pig samples.
|Tested Reactivity||Human, Rat, Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GORASP2 fusion protein Ag17607|
|Full Name||golgi reassembly stacking protein 2, 55kDa|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC007770|
|Gene ID (NCBI)||26003|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.
J Cell Biol
Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Front Cell Dev Biol
Long-term culture of patient-derived cardiac organoids recapitulated Duchenne muscular dystrophy cardiomyopathy and disease progression
Cell Death Dis
A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (01-11-2020)
This antibody can be used for detecting the Golgi localisation of endogenous Gorasp2.