GORASP2 Monoclonal antibody

GORASP2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C9A3

Cat no : 66627-1-Ig

Synonyms

Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59



Tested Applications

Positive WB detected inA549 cells, pig brain tissue, rat brain tissue, NCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66627-1-Ig targets GORASP2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Rat, Pig samples.

Tested Reactivity Human, Rat, Pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GORASP2 fusion protein Ag17607
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight 55 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC007770
Gene symbol GORASP2
Gene ID (NCBI) 26003
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protocols

Product Specific Protocols
WB protocol for GORASP2 antibody 66627-1-IgDownload protocol
IHC protocol for GORASP2 antibody 66627-1-IgDownload protocol
IF protocol for GORASP2 antibody 66627-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell Death Dis

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Authors - Peng Wang
humanWB,IF

J Cell Biol

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

Authors - Yijun Zhang
humanWB,IF

Mol Cell

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Authors - Julian Nüchel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (01-11-2020)

This antibody can be used for detecting the Golgi localisation of endogenous Gorasp2.

  • Applications: Immunofluorescence,