|Positive WB detected in||A431 cells, HepG2 cells, mouse heart tissue, mouse spleen tissue, HEK-293 cells, mouse brain, rat brain|
|Positive IHC detected in||human breast cancer tissue, human skin tissue, human liver tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14800-1-AP targets GOT2 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GOT2 fusion protein Ag6514|
|Full Name||glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||43-45 kDa|
|GenBank accession number||BC000525|
|Gene ID (NCBI)||2806|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.
Am J Respir Cell Mol Biol
Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Cell Stem Cell
Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.
J Proteome Res
Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.
J Biol Chem
The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.
J Cell Mol Med
HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.
Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors.