• Featured Product
  • KD/KO Validated

GOT2 Polyclonal antibody

GOT2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14800-1-AP


FABPpm, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

Tested Applications

Positive WB detected inA431 cells, HepG2 cells, mouse heart tissue, mouse spleen tissue, HEK-293 cells, mouse brain, rat brain
Positive IHC detected inhuman breast cancer tissue, human skin tissue, rat brain tissue, human liver tissue, rat heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14800-1-AP targets GOT2 in WB,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOT2 fusion protein Ag6514
Full Name glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC000525
Gene Symbol GOT2
Gene ID (NCBI) 2806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.


Product Specific Protocols
WB protocol for GOT2 antibody 14800-1-APDownload protocol
IHC protocol for GOT2 antibody 14800-1-APDownload protocol
IF protocol for GOT2 antibody 14800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Stem Cell

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.

Authors - Le Qi
  • KO Validated

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen

Cell Metab

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Authors - Yi-Ping Wang

Cell Res

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Authors - Wei-Feng Cai
  • KD Validated

Nat Commun

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Authors - Yun-Qian Wang

Nat Commun

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Authors - Maren Feist