• Featured Product
  • KD/KO Validated

GOT2 Polyclonal antibody

GOT2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14800-1-AP

Synonyms

FABP 1, FABPpm, Fatty acid binding protein, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A



Tested Applications

Positive WB detected inA431 cells, HepG2 cells, mouse heart tissue, mouse spleen tissue, HEK-293 cells, mouse brain, rat brain
Positive IHC detected inhuman breast cancer tissue, human skin tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14800-1-AP targets GOT2 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOT2 fusion protein Ag6514
Full Name glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Calculated molecular weight 47 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC000525
Gene symbol GOT2
Gene ID (NCBI) 2806
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Protocols

Product Specific Protocols
WB protocol for GOT2 antibody 14800-1-APDownload protocol
IHC protocol for GOT2 antibody 14800-1-APDownload protocol
IF protocol for GOT2 antibody 14800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Respir Cell Mol Biol

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Authors - Robert B Hamanaka
  • KD Validated
mouseWB

Cell Stem Cell

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.

Authors - Le Qi
  • KO Validated
human, mouseWB

J Proteome Res

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Authors - Shizhen Qin
humanWB

J Biol Chem

The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.

Authors - Victor Vitvitsky
  • KD Validated
humanWB

J Cell Mol Med

HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Authors - Wenzhu Li
humanWB

Oncotarget

Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors.

Authors - Satoshi Kitazawa