GPX4 Polyclonal antibody

GPX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14432-1-AP

Synonyms

GPX4



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Sample-dependent, check data in validation data gallery

Product Information

14432-1-AP targets GPX4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPX4 fusion protein Ag5809
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Calculated molecular weight 22 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC022071
Gene symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Protocols

Product Specific Protocols
WB protocol for GPX4 antibody 14432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Differ

Loss of ferroportin induces memory impairment by promoting ferroptosis in Alzheimer's disease.

Authors - Wen-Dai Bao
ratWB

Redox Biol

Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.

Authors - Caiying Liu
humanWB

Theranostics

HIF-1-induced mitochondrial ribosome protein L52: a mechanism for breast cancer cellular adaptation and metastatic initiation in response to hypoxia.

Authors - Xinyan Li
mouseWB

Cell Chem Biol

3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.

Authors - Chenwen Shao
mouseWB

Br J Pharmacol

Targeted inhibition of Rev-erbα/β limits ferroptosis to ameliorate folic acid-induced acute kidney injury.

Authors - Lianxia Guo
ratWB,IHC

J Nanobiotechnology

miR-124-3p delivered by exosomes from heme oxygenase-1 modified bone marrow mesenchymal stem cells inhibits ferroptosis to attenuate ischemia-reperfusion injury in steatotic grafts.

Authors - Longlong Wu