GPX4 Polyclonal antibody

GPX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14432-1-AP

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx

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Validation Data Gallery

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  WB analysis using 14432-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14432-1-AP (GPX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

14432-1-AP targets GPX4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPX4 fusion protein Ag5809
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Calculated molecular weight: 22 kDa
• Observed molecular weight: 20-23 kDa
• GenBank accession number: BC022071
• Gene symbol: GPX4
• Gene ID (NCBI): 2879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publications

Species	Application	Title
mouse	WB	Am J Transl Res; Effects of autophagy on the spermatogonia of chronic hypoxic mice.; Authors - Shaoming Zhu
mouse	WB	Hepatol Commun; Adipose-Specific Lipin-1 Overexpression Renders Hepatic Ferroptosis and Exacerbates Alcoholic Steatohepatitis in Mice.; Authors - Zhou Zhou
rat	WB	Am J Physiol Cell Physiol; IGF-IIRα is a novel stress-inducible contributor to cardiac damage underpinning doxorubicin-induced oxidative stress and perturbed mitochondrial autophagy.; Authors - Sudhir Pandey
mouse	WB,IF	J Inflamm (Lond); Ferroptosis inhibitor alleviates Radiation-induced lung fibrosis (RILF) via down-regulation of TGF-β1.; Authors - Xuan Li
rat	IF	Am J Physiol Cell Physiol; Choroid plexus glutathione peroxidases are instrumental in protecting the brain fluid environment from hydroperoxides during postnatal development.; Authors - Elodie Saudrais
human	WB	Oxid Med Cell Longev; IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer.; Authors - Lei Zhang