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GPX4 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 14432-1-AP

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Con: 31 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

GPX4 Antibody WB mouse kidney tissue 14432-1-AP

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14432-1-AP(GPX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

GPX4 Antibody WB mouse testis tissue 14432-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 14432-1-AP (GPX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

GPX4 Antibody IHC human testis tissue 14432-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14432-1-AP (GPX4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

GPX4 Antibody IHC human testis tissue 14432-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14432-1-AP (GPX4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

GPX4 Antibody IF human kidney tissue 14432-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human kidney tissue using 14432-1-AP (GPX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

GPX4 Antibody IP mouse kidney tissue 14432-1-AP

IP Result of anti-GPX4 (IP:14432-1-AP, 4ug; Detection:14432-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse testis tissue, mouse kidney tissue, human testis tissue, rat testis tissue
Positive IP detected in:
mouse kidney tissue
Positive IHC detected in:
human testis tissue, mouse testis tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human kidney tissue
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GPX4 fusion protein Ag5809
Full name:
glutathione peroxidase 4 (phospholipid hydroperoxidase)
Calculated molecular weight:
22 kDa
Observed molecular weight:
20 kDa-23 kDa
GenBank accession number:
BC022071
Gene ID (NCBI):
2879
Gene symbol
GPX4
Synonyms
Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx

Background

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers(PMID:17630701).It presents primarily in testis.


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