GPX4 Polyclonal antibody

GPX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

97

Cat no : 14432-1-AP

Synonyms

GPX4

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Validation Data Gallery

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  WB analysis using 14432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14432-1-AP (GPX4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 88 publications below
• IHC: See 21 publications below
• IF: See 12 publications below
• CoIP: See 1 publications below

Product Information

14432-1-AP targets GPX4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPX4 fusion protein Ag5809
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Calculated molecular weight: 22 kDa
• Observed molecular weight: 20-23 kDa
• GenBank accession number: BC022071
• Gene symbol: GPX4
• Gene ID (NCBI): 2879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publications

Species	Application	Title
mouse	WB	Cell Death Differ; Loss of ferroportin induces memory impairment by promoting ferroptosis in Alzheimer's disease.; Authors - Wen-Dai Bao
rat	WB	Redox Biol; Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.; Authors - Caiying Liu
human	WB	Theranostics; HIF-1-induced mitochondrial ribosome protein L52: a mechanism for breast cancer cellular adaptation and metastatic initiation in response to hypoxia.; Authors - Xinyan Li
mouse	WB	Cell Chem Biol; 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.; Authors - Chenwen Shao
mouse	WB	Br J Pharmacol; Targeted inhibition of Rev-erbα/β limits ferroptosis to ameliorate folic acid-induced acute kidney injury.; Authors - Lianxia Guo
rat	WB,IHC	J Nanobiotechnology; miR-124-3p delivered by exosomes from heme oxygenase-1 modified bone marrow mesenchymal stem cells inhibits ferroptosis to attenuate ischemia-reperfusion injury in steatotic grafts.; Authors - Longlong Wu