GPX4 Polyclonal antibody

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  WB analysis using 14432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14432-1-AP (GPX4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14432-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPX4 antibody (14432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

GPX4 Polyclonal Antibody for WB, IF, ELISA

Cat No. 14432-1-AP

Publications(147)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

GPX4

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 134 publications below
• IHC: See 25 publications below
• IF: See 18 publications below
• CoIP: See 2 publications below

Product Information

14432-1-AP targets GPX4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPX4 fusion protein Ag5809
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC022071
• Gene Symbol: GPX4
• Gene ID (NCBI): 2879
• RRID: AB_10898356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36969
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Drug Resist Updat; Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance; Authors - Xu Lin
mouse	WB	Biomaterials; Tumor microenvironment-initiated lipid redox cycling for efficient triple-negative breast cancer therapy; Authors - Tian-Jiao Zhou
chicken	WB	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
mouse	IHC	Drug Des Devel Ther; Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides; Authors - Xuedong Yin
mouse	WB	Drug Des Devel Ther; Ketamine Attenuates Airway Inflammation via Inducing Inflammatory Cells Apoptosis and Activating Nrf2 Pathway in a Mixed-Granulocytic Murine Asthma Model; Authors - Shilin Xiao
mouse	WB	Mater Today Bio; In situ injectable hydrogel-loaded drugs induce anti-tumor immune responses in melanoma immunochemotherapy.; Authors - Jiehan Li