Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, rat stomach tissue, rat brain tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10747-2-AP targets GRASP65,GORASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1054 Product name: Recombinant human GRASP65,GORASP1 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-215 aa of BC008928 Sequence: MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEPSSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCRESGMWHWLWVSTPDPNSAPQLPQEATWHPTTFCSTTWCPTT Predict reactive species |
| Full Name | golgi reassembly stacking protein 1, 65kDa |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC008928 |
| Gene Symbol | GRASP65 |
| Gene ID (NCBI) | 64689 |
| RRID | AB_2113204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. (refer to Wikipedia)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GRASP65,GORASP1 antibody 10747-2-AP | Download protocol |
| IHC protocol for GRASP65,GORASP1 antibody 10747-2-AP | Download protocol |
| WB protocol for GRASP65,GORASP1 antibody 10747-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
GRASP65 (GORASP1) polyclonal antibody (Cat# 10747-2-AP) has 14 citations across journals including Cell Death Dis, Molecular Medicine, Antioxidants & Redox Signaling, CNS Neuroscience & Therapeutics, Scientific Reports, iScience, Journal of Cellular Biochemistry, Prostaglandins & Other Lipid Mediators, Biochemical & Biophysical Research Communications, Methods in Molecular Biology, eLife, and Pharmaceutics. Research fields span Golgi apparatus structure/stress, apoptosis, inflammation, neurodegeneration (Parkinson's disease), renal and hepatic injury, endotoxemia, depression, calcium signaling, lipid metabolism, and male infertility.
| Species | Application | Title |
|---|---|---|
Cell Death Dis GOLPH3 promotes endotoxemia-induced liver and kidney injury through Golgi stress-mediated apoptosis and inflammatory response | ||
Mol Med The role of Dymeclin in chronic unpredictable mild stress-induced depression: maintaining the Golgi apparatus structure and regulating NLRP3 inflammasome activation. | ||
Pharmaceutics Tjap1/Pilt Is a cis-Golgi-Associated Protein Required for Golgi Integrity and Normal Drug Transporter Expression in Brain Microvascular Endothelial Cells In Vitro. | ||
Antioxid Redox Signal Thioredoxin-1 Inhibits Golgi Stress Induced by Methyl-4-Phenyl-1,2,3, 6-Tetrahydropyridine. | ||
CNS Neurosci Ther SIX2-Mediated Microglial M2 Polarization and Exosomal miR-3470b Delivery Protect Dopaminergic Neurons in Parkinson's Disease. | ||
Sci Rep GDNF improves the cognitive ability of PD mice by promoting glycosylation and membrane distribution of DAT |
