GREB1 Recombinant antibody

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  WB analysis of MCF-7 using 85189-4-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 85189-4-RR

  T-47D cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85189-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85189-4-RR against Human GREB1 were performed. The affinity constant is 45.5 nM.

GREB1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85189-4-RR

Clone No.242882D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242882D11, GREB1 protein, KIAA0575, Protein GREB1

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Tested Applications

• Positive WB detected in: T-47D cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85189-4-RR targets GREB1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GREB1 fusion protein Ag29908
• Full Name: GREB1 protein
• Calculated Molecular Weight: 216 kDa
• Observed Molecular Weight: 216-240 kDa
• GenBank Accession Number: NM_014668
• Gene Symbol: GREB1
• Gene ID (NCBI): 9687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q4ZG55
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.