GSK3B Polyclonal antibody

• Featured Product
• KD/KO Validated

GSK3B Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

127

Reviews (1)

Cat no : 22104-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta, GSK3β

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Validation Data Gallery

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  WB analysis of A549 using 22104-1-AP

  WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

• 

  WB analysis using 22104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 22104-1-AP

  IP Result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GSK3B antibody 22104-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, LNCaP cells, mouse brain tissue, mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, rat thymus tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human prostate hyperplasia tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 119 publications below
• IHC: See 7 publications below
• IF: See 1 publications below

Product Information

The immunogen of 22104-1-AP is GSK3B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: hamster, human, mouse, pig, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSK3B fusion protein Ag17320
• Full Name: glycogen synthase kinase 3 beta
• Calculated molecular weight: 433 aa, 48 kDa
• Observed molecular weight: 46-48 kDa
• GenBank accession number: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution. This antibody recognize the C-terminal of GSK3B.

Publications

Species	Application	Title
human	WB	Int J Mol Med; ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.; Authors - Wei Li
mouse	WB	Front Cell Neurosci; HDAC1 Silence Promotes Neuroprotective Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Mouse Model of Traumatic Brain Injury via PI3K/AKT Pathway.; Authors - Ling Xu
human	WB	Sci Rep; PMID: 30816192; Authors - Lin Zhu
mouse	WB	Phytother Res; N-(3-methoxybenzyl)-(9Z,12Z,15Z)-octadecatrienamide promotes bone formation via the canonical Wnt/β-catenin signaling pathway.; Authors - Ting Wang
human	WB	FASEB J; Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.; Authors - Wei Peng
human	WB	Cancer Med; A novel tumor suppressor SPINK5 targets Wnt/β-catenin signaling pathway in esophageal cancer.; Authors - Qian Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

HEK293 lysates - 10ug total protein. Blocked in 5% BSA before primary antibody (1:1000) overnight at 4 degrees. Anti-rabbit HRP (1:10,000) for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

GSK3B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:22104-1-AP)