GSK3B Polyclonal antibody

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  WB analysis of A549 using 22104-1-AP

  WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

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  WB analysis using 22104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22104-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22104-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 22104-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 22104-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22104-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22104-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IP experiment of mouse brain using 22104-1-AP

  IP result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GSK3B antibody 22104-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22104-1-AP

Publications(388)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

GSK3 beta, GSK3A B, GSK-3 beta, GSK 3 beta, EC:2.7.11.26

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse brain tissue, mouse kidney tissue, mouse ovary tissue, LNCaP cells, mouse testis tissue, rat brain tissue, PC-3 cells, mouse thymus tissue, rat thymus tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human prostate hyperplasia tissue, human testis tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 371 publications below
• IHC: See 16 publications below
• IF: See 12 publications below
• IP: See 7 publications below
• CoIP: See 6 publications below
• RIP: See 1 publications below

Product Information

22104-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSK3B fusion protein Ag17320
• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 46-48 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• RRID: AB_2878997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49841
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution. This antibody recognize the C-terminal of GSK3B.

Publications

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha
human,mouse	WB	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
human	WB	Mol Cancer; Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1.; Authors - Shan Xing
human	WB	Cell Rep Med; Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition; Authors - Yu Chang
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
zebrafish	WB	Nat Commun; ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.; Authors - Xiaoyun Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

HEK293 lysates - 10ug total protein. Blocked in 5% BSA before primary antibody (1:1000) overnight at 4 degrees. Anti-rabbit HRP (1:10,000) for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

GSK3B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:22104-1-AP)