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GSK3B Antibody 89 Publications

Rabbit Polyclonal| Catalog number: 22104-1-AP

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Con: 62 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

GSK3B Antibody WB A549 cells 22104-1-AP

WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells

GSK3B Antibody WB HeLa cells 22104-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

GSK3B Antibody IP mouse brain tissue 22104-1-AP

IP Result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

GSK3B Antibody IF MCF-7 cells 22104-1-AP

Immunofluorescent analysis of MCF-7 cells, using GSK3B antibody 22104-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

GSK3B Antibody IHC human lung cancer tissue 22104-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP( GSK3B Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

GSK3B Antibody IHC human lung cancer tissue 22104-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP( GSK3B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, hamster, pig
Positive WB detected in:
HeLa cells, A549 cells, LNCaP cells, mouse brain tissue, mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, rat thymus tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human lung cancer tissue, human prostate hyperplasia tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GSK3B fusion protein Ag17320
Full name:
glycogen synthase kinase 3 beta
Calculated molecular weight:
433aa,48 kDa
Observed molecular weight:
46-48 kDa
GenBank accession number:
BC000251
Gene ID (NCBI):
2932
Gene symbol
GSK3B
Synonyms
GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.


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