|Positive WB detected in||HeLa cells, A549 cells, LNCaP cells, mouse brain tissue, mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, rat thymus tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human lung cancer tissue, human prostate hyperplasia tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22104-1-AP is GSK3B Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat, swine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GSK3B fusion protein Ag17320|
|Full Name||glycogen synthase kinase 3 beta|
|Calculated molecular weight||433 aa, 48 kDa|
|Observed molecular weight||46-48 kDa|
|GenBank accession number||BC000251|
|Gene ID (NCBI)||2932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.
|Product Specific Protocols|
|WB protocol for GSK3B antibody 22104-1-AP||Download protocol|
|IHC protocol for GSK3B antibody 22104-1-AP||Download protocol|
|IF protocol for GSK3B antibody 22104-1-AP||Download protocol|
|IP protocol for GSK3B antibody 22104-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (12-15-2020)
HEK293 lysates - 10ug total protein. Blocked in 5% BSA before primary antibody (1:1000) overnight at 4 degrees. Anti-rabbit HRP (1:10,000) for 1 hour at RT.