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GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22104-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta



Tested Applications

Positive WB detected inHeLa cells, A549 cells, LNCaP cells, mouse brain tissue, mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, rat thymus tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human prostate hyperplasia tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22104-1-AP is GSK3B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSK3B fusion protein Ag17320
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight 46-48 kDa
GenBank accession numberBC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 22104-1-APDownload protocol
IHC protocol for GSK3B antibody 22104-1-APDownload protocol
IF protocol for GSK3B antibody 22104-1-APDownload protocol
IP protocol for GSK3B antibody 22104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.

Authors - Wei Li
mouseWB

Front Cell Neurosci

HDAC1 Silence Promotes Neuroprotective Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Mouse Model of Traumatic Brain Injury via PI3K/AKT Pathway.

Authors - Ling Xu
humanWB

Sci Rep

PMID: 30816192

Authors - Lin Zhu
mouseWB

Phytother Res

N-(3-methoxybenzyl)-(9Z,12Z,15Z)-octadecatrienamide promotes bone formation via the canonical Wnt/β-catenin signaling pathway.

Authors - Ting Wang
humanWB

FASEB J

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

Authors - Wei Peng
humanWB

Cancer Med

A novel tumor suppressor SPINK5 targets Wnt/β-catenin signaling pathway in esophageal cancer.

Authors - Qian Wang