Various lysates were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14475-1-AP
WB result of GSTA1 antibody (14475-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTA1 transfected HepG2 cells.
WB result of GSTA1 antibody (14475-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTA1 transfected HepG2 cells.
WB analysis of human kidney using 14475-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human kidney tissue were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14475-1-AP
IP result of anti-GSTA1 (IP:14475-1-AP, 3ug; Detection:14475-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IP result of anti-GSTA1 (IP:14475-1-AP, 3ug; Detection:14475-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human lung cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.
Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.
IF Staining of HepG2 using 14475-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTA1 antibody (14475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTA1 antibody (14475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse kidney tissue, human kidney tissue, HepG2 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IP detected in
mouse liver tissue
Positive IHC detected in
human lung cancer tissue, human liver tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
GSTA1 encodes one of the GST alpha isozymes, belongs to GST family, which is mainly expressed in the liver. GST can improve the detoxification of organism by enzymatic and non-enzymatic methods, and has anti-mutagenic and anti-tumor effects. Studies have shown that overexpression of GSTA1 can promote the development of lung cancer cells, so the expression level of GSTA1 can be used to predict the degree of malignancy of lung cancer tissues. To some extent, GSTA1 is regarded as a potential lung cancer candidate targets.
Various lysates were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14475-1-AP
WB result of GSTA1 antibody (14475-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTA1 transfected HepG2 cells.
WB analysis of human kidney using 14475-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.
IHC staining of human kidney using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.
IHC staining of human kidney using 14475-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.
IP Figures
IP experiment of mouse liver using 14475-1-AP
IP result of anti-GSTA1 (IP:14475-1-AP, 3ug; Detection:14475-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IF/ICC Figures
IF Staining of HepG2 using 14475-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTA1 antibody (14475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
GSTA1 Polyclonal antibody
Catalog Number
14475-1-AP
Citations
19
Dilutions
WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet