Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, human kidney tissue, HepG2 cells, mouse liver tissue, rat kidney tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human lung cancer tissue, human liver tissue, human colon cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14475-1-AP targets GSTA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GSTA1 fusion protein Ag5849|
|Full Name||glutathione S-transferase alpha 1|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC053578|
|Gene ID (NCBI)||2938|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GSTA1 encodes one of the GST alpha isozymes, belongs to GST family, which is mainly expressed in the liver. GST can improve the detoxification of organism by enzymatic and non-enzymatic methods, and has anti-mutagenic and anti-tumor effects. Studies have shown that overexpression of GSTA1 can promote the development of lung cancer cells, so the expression level of GSTA1 can be used to predict the degree of malignancy of lung cancer tissues. To some extent, GSTA1 is regarded as a potential lung cancer candidate targets.
|Product Specific Protocols|
|WB protocol for GSTA1 antibody 14475-1-AP||Download protocol|
|IHC protocol for GSTA1 antibody 14475-1-AP||Download protocol|
|IF protocol for GSTA1 antibody 14475-1-AP||Download protocol|
|IP protocol for GSTA1 antibody 14475-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.
J Gerontol A Biol Sci Med Sci
Food Chem Toxicol
l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.
Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells.
Appl Physiol Nutr Metab
Rice protein suppresses 4-hydroxy-2-nonenal-induced inflammation owing to methionine availability.