HAUS3 Polyclonal antibody, PBS Only

• 

  WB analysis of HepG2 using 18991-1-AP (same clone as 18991-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18991-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18991-1-AP (same clone as 18991-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18991-1-AP (same clone as 18991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18991-1-AP (same clone as 18991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18991-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18991-1-AP (same clone as 18991-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18991-1-AP (HAUS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18991-1-PBS in a different storage buffer formulation.

HAUS3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18991-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

---

Product Information

18991-1-PBS targets HAUS3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chromosome 4 open reading frame 15
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: NM_024511
• Gene Symbol: HAUS3
• Gene ID (NCBI): 79441
• RRID: AB_2301967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q68CZ6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HAUS3, also named as C4orf15, belongs to the HAUS3 family. It is Required for mitotic centrosome and spindle integrity. HAUS3 is a component of the HAUS augmin-like complex which regulates mitotic spindle assembly and centrosome integrity and is required for completion of cytokinesis. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly.