HAUS3 Polyclonal antibody

HAUS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18991-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C4orf15, HAUS augmin-like complex subunit 3, IT1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 18991-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 18991-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 18991-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 18991-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human urothelial carcinoma using 18991-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18991-1-AP (HAUS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 18991-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HAUS3 antibody (18991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 18991-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAUS3 antibody (18991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells
Positive IHC detected in	human urothelial carcinoma tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18991-1-AP targets HAUS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	chromosome 4 open reading frame 15
Calculated Molecular Weight	70 kDa
Observed Molecular Weight	62-65 kDa
GenBank Accession Number	NM_024511
Gene Symbol	HAUS3
Gene ID (NCBI)	79441
RRID	AB_2301967
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q68CZ6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAUS3, also named as C4orf15, belongs to the HAUS3 family. It is Required for mitotic centrosome and spindle integrity. HAUS3 is a component of the HAUS augmin-like complex which regulates mitotic spindle assembly and centrosome integrity and is required for completion of cytokinesis. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly.

Protocols

Product Specific Protocols
IF protocol for HAUS3 antibody 18991-1-AP	Download protocol
IHC protocol for HAUS3 antibody 18991-1-AP	Download protocol
WB protocol for HAUS3 antibody 18991-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The HAUS3 Polyclonal antibody (Cat# 18991-1-AP) has 1 citation. It was used in Western Blot in a study published in Journal of Cell Biology (2022), which investigated the reconstitution and mechanistic dissection of the human microtubule branching machinery. The research field is cell biology, specifically mitotic spindle assembly and centrosome integrity.