HAUS3 Polyclonal antibody

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  WB analysis of HepG2 using 18991-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18991-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18991-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 18991-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human urothelial carcinoma using 18991-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18991-1-AP (HAUS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18991-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HAUS3 antibody (18991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 18991-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAUS3 antibody (18991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

HAUS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18991-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C4orf15, HAUS augmin-like complex subunit 3, IT1

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human urothelial carcinoma tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18991-1-AP targets HAUS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chromosome 4 open reading frame 15
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: NM_024511
• Gene Symbol: HAUS3
• Gene ID (NCBI): 79441
• RRID: AB_2301967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q68CZ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAUS3, also named as C4orf15, belongs to the HAUS3 family. It is Required for mitotic centrosome and spindle integrity. HAUS3 is a component of the HAUS augmin-like complex which regulates mitotic spindle assembly and centrosome integrity and is required for completion of cytokinesis. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Cell Biol; Reconstitution and mechanistic dissection of the human microtubule branching machinery.; Authors - Yaqian Zhang