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HDAC1 Polyclonal antibody
HDAC1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10197-1-AP
Validation Data Gallery
|Positive WB detected in||C6 cells, HeLa cells, HepG2 cells, mouse testis tissue, Jurkat cells, K-562 cells, NIH/3T3 cells|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human pancreas cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, HeLa cells|
|Positive FC detected in||HeLa cells, SH-SY5Y cells|
|Western Blot (WB)||WB : 1:8000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10197-1-AP targets HDAC1 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HDAC1 fusion protein Ag0256|
|Full Name||histone deacetylase 1|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC000301|
|Gene ID (NCBI)||3065|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HDAC1 poly https://www.ptglab.com/products/HDAC1-Antibody-10197-1-AP.htm
Histone Deacetylase 1 (HDAC1) is a nuclear localized class I histone deacetylase that plays a role in the regulation of gene expression, mainly by repression of gene activity. Additionally, HDAC can mediate deacetylation of a subset of non-histone proteins, leading to their degradation. HDAC1 activity has an impact on cell growth, proliferation, and death.
1. What is the molecular weight of HDAC1?
The molecular size of HDAC1 is 60 kDa.
2. What is the subcellular localization of HDAC1?
Unlike some HDACs, HDAC1 is present entirely in the nucleus. Our HDAC1 antibody has been broadly tested for IF/ICC.
3. I cannot detect an HDAC1 specific signal in my sample during western blotting.
Make sure that you efficiently extract nuclear proteins during your sample preparation. Some lysis buffers (e.g., based on Triton X-100) may not extract nuclear proteins. We recommend using RIPA buffer (https://www.ptglab.com/support/protocols/). We highly recommend using nuclear loading control antibodies, such as lamin B1 or PCNA (https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/). Alternatively, you may consider performing cell fractionation.
4. How do I perform chromatin immunoprecipitation (ChiP) with the HDAC1 antibody?
We recommend using our standard chromatin immunoprecipitation protocol (https://www.ptglab.com/media/2708/web_chip-protocol.pdf).
5. Is HDAC1 post-translationally modified?
HDAC1 is a protein deacetylase but itself can also be a subject of post-translational modifications, including phosphorylation, acetylation, ubiquitination, SUMOylation, nitrosylation, and carbonylation (PMID: 21197454).
6. What is the role of HDAC1 in cancer?
The acetylation and deacetylation of histones play an important part in the epigenetic regulation of gene expression. Alterations in the balance between these two opposing processes changes chromosome remodeling and increased deacetylation leads to gene silencing. HDAC1 levels are increased in many cancer cell types and the role of HDAC1 in cancer is connected not only to histone deacetylation but also to the deacetylation of other proteins, such as Rb family proteins, estrogen receptors, and p53 protein (PMID: 19383284).
|Product Specific Protocols|
|WB protocol for HDAC1 antibody 10197-1-AP||Download protocol|
|IHC protocol for HDAC1 antibody 10197-1-AP||Download protocol|
|IF protocol for HDAC1 antibody 10197-1-AP||Download protocol|
|IP protocol for HDAC1 antibody 10197-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Biol Chem
Connexin32 regulates hepatoma cell metastasis and proliferation via the p53 and Akt pathways.
Clin Transl Med
EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.
J Cell Mol Med
Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.
Cell Death Dis
HDAC1 inhibition ameliorates TDP-43-induced cell death in vitro and in vivo.
Cell Death Dis
Crosstalk between cardiomyocytes and noncardiomyocytes is essential to prevent cardiomyocyte apoptosis induced by proteasome inhibition.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Dipen (Verified Customer) (07-20-2020)
Great antibody for Western blotting.
Uxoa (Verified Customer) (02-19-2020)
HDAC1 in human primary fibroblasts untreated vs treated. Cells fixed with 4% PFA 15 min RT. HDAC1 incubation 1:50 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit Alexa fluor 555 1:500 1h RT.