Validation Data Gallery
|Positive WB detected in||A549 cells, HEK-293 cells, HeLa cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human tonsillitis tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15240-1-AP targets HLA class I ABC in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||HLA class I ABC fusion protein Ag7370|
|Full Name||major histocompatibility complex, class I, A|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||44 kDa|
|GenBank accession number||BC003069|
|Gene ID (NCBI)||3105|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
|Product Specific Protocols|
|WB protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|IHC protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|IF protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|IP protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Loss of Fas expression and high expression of HLA-E promoting the immune escape of early colorectal cancer cells.
The prognostic impact of human leukocyte antigen (HLA) class I antigen abnormalities in salivary gland cancer. A clinicopathological study of 288 cases.
Noncoding RNA 886 alleviates tumor cellular immunological rejection in host C57BL/C mice.
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Activation of Toll-like receptor 7 signaling in labial salivary glands of primary Sjögren's syndrome patients.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Patricia (Verified Customer) (02-25-2020)
I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.