Validation Data Gallery
|Positive WB detected in
|A549 cells, HEK-293 cells, HeLa cells, HepG2 cells
|Positive IP detected in
|Positive IHC detected in
|human tonsillitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15240-1-AP targets HLA class I ABC in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|HLA class I ABC fusion protein Ag7370
|major histocompatibility complex, class I, A
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
|Product Specific Protocols
|WB protocol for HLA class I ABC antibody 15240-1-AP
|IHC protocol for HLA class I ABC antibody 15240-1-AP
|IF protocol for HLA class I ABC antibody 15240-1-AP
|IP protocol for HLA class I ABC antibody 15240-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Fabien (Verified Customer) (11-04-2022)
The antibody works great for western-blot.
Patricia (Verified Customer) (02-25-2020)
I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.