HLA class I ABC Polyclonal antibody

HLA class I ABC Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 15240-1-AP


HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15240-1-AP targets HLA class I ABC in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7370
Full Name major histocompatibility complex, class I, A
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC003069
Gene Symbol HLA-A
Gene ID (NCBI) 3105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)


Product Specific Protocols
WB protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IHC protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IF protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IP protocol for HLA class I ABC antibody 15240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Fabien (Verified Customer) (11-04-2022)

The antibody works great for western-blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Jy cells

Patricia (Verified Customer) (02-25-2020)

I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000 for WB; 4-10 ug/mL IP
  • Cell Tissue Type: Ovarian cancer