HNF1A Polyclonal antibody

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  WB analysis of rat liver using 22426-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22426-1-AP (HNF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 22426-1-AP

  WB result of HNF1A antibody (22426-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNF1A transfected HuH-7 cells.

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  WB analysis of SMMC-7721 using 22426-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 22426-1-AP (HNF1A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 22426-1-AP

  IP result of anti-HNF1A (IP:22426-1-AP, 4ug; Detection:22426-1-AP 1:800) with mouse liver tissue lysate 3600ug.

• Featured Product
• KD/KO Validated

HNF1A Polyclonal Antibody for WB, IP, ELISA

Cat No. 22426-1-AP

Publications(13)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, chIP, ELISA

Synonyms

Hepatocyte nuclear factor 1-alpha, HNF 1 alpha, HNF 1A, HNF1, HNF1 homeobox A

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Tested Applications

• Positive WB detected in: rat liver tissue, SMMC-7721 cells, HuH-7 cells
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 2 publications below

Product Information

22426-1-AP targets HNF1A in WB, IHC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNF1A fusion protein Ag18188
• Full Name: HNF1 homeobox A
• Calculated Molecular Weight: 631 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC104910
• Gene Symbol: HNF1A
• Gene ID (NCBI): 6927
• RRID: AB_2879104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P20823
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
human	ChIP	Mol Ther Nucleic Acids; The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2.; Authors - Guifang Du
rat	WB	Acta Pharmacol Sin; Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats.; Authors - Zhen-Li Su
human	WB	Chem Biol Interact; 6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.; Authors - Yuanhang Ma ; KD Validated
human	WB,IHC,CoIP	J Exp Clin Cancer Res; ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.; Authors - Shuohui Dong
	CoIP	Cell Death Dis; CBP/p300 HAT maintains the gene network critical for β cell identity and functional maturity.; Authors - Linlin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (07-05-2023)

Works with 1X TBST for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Liver tissue