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- KD/KO Validated
HNF1A Polyclonal antibody
HNF1A Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IP, IHC, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 22426-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat liver tissue, SMMC-7721 cells |
Positive IP detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IHC | See 1 publications below |
CoIP | See 3 publications below |
ChIP | See 2 publications below |
Product Information
The immunogen of 22426-1-AP is HNF1A Fusion Protein expressed in E. coli.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HNF1A fusion protein Ag18188 |
Full Name | HNF1 homeobox A |
Calculated molecular weight | 631 aa, 67 kDa |
Observed molecular weight | 67 kDa |
GenBank accession number | BC104910 |
Gene symbol | HNF1A |
Gene ID (NCBI) | 6927 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Protocols
Product Specific Protocols | |
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WB protocol for HNF1A antibody 22426-1-AP | Download protocol |
IP protocol for HNF1A antibody 22426-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theranostics Small Heterodimer Partner Regulates Circadian Cytochromes p450 and Drug-Induced Hepatotoxicity. | ||
FEBS Lett Hepatocyte nuclear factor 1 alpha (HNF1A) regulates transcription of O-GlcNAc transferase in a negative feedback mechanism. | ||
Cell Death Dis CBP/p300 HAT maintains the gene network critical for β cell identity and functional maturity. | ||
Food Nutr Res Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism. | ||
J Exp Clin Cancer Res ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer. | ||
Chem Biol Interact 6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.
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