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HNF1A Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 22426-1-AP

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Con: 84 μg/150 μl

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Tested Applications

Applications:
WB, IP, ELISA
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Images See all

HNF1A Antibody WB rat liver tissue 22426-1-AP

rat liver tissue were subjected to SDS PAGE followed by western blot with 22426-1-AP(HNF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HNF1A Antibody IP mouse liver tissue 22426-1-AP

IP Result of anti-HNF1A (IP:22426-1-AP, 4ug; Detection:22426-1-AP 1:800) with mouse liver tissue lysate 3600ug.

Species specificity:
human, rat, mouse
Positive WB detected in:
rat liver tissue, SMMC-7721 cells
Positive IP detected in:
mouse liver tissue
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen Affinity purified
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HNF1A fusion protein Ag18188
Full name:
HNF1 homeobox A
Calculated molecular weight:
631aa,67 kDa
Observed molecular weight:
67 kDa
GenBank accession number:
BC104910
Gene ID (NCBI):
6927
Gene symbol
HNF1A
Synonyms
HNF 1 alpha, HNF 1A, HNF1, HNF1 homeobox A, HNF1A, LFB1, MODY3, TCF 1, TCF1, Transcription factor 1

Background

HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.


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