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  • KD/KO Validated

HNF1A Polyclonal antibody

HNF1A Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 22426-1-AP

Synonyms

HNF 1 alpha, HNF 1A, HNF1, HNF1 homeobox A, HNF1A, Hnf1α, LFB1, MODY3, TCF 1, TCF1, Transcription factor 1



Tested Applications

Positive WB detected inrat liver tissue, SMMC-7721 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22426-1-AP is HNF1A Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNF1A fusion protein Ag18188
Full Name HNF1 homeobox A
Calculated molecular weight 631 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC104910
Gene symbol HNF1A
Gene ID (NCBI) 6927
RRIDAB_2879104
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Protocols

Product Specific Protocols
WB protocol for HNF1A antibody 22426-1-APDownload protocol
IP protocol for HNF1A antibody 22426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.

Authors - Shuaishuai Teng
humanChIP

Mol Ther Nucleic Acids

The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2.

Authors - Guifang Du
ratWB

Acta Pharmacol Sin

Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats.

Authors - Zhen-Li Su
humanWB

Chem Biol Interact

6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.

Authors - Yuanhang Ma
  • KD Validated
humanWB,IHC,CoIP

J Exp Clin Cancer Res

ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.

Authors - Shuohui Dong
CoIP

Cell Death Dis

CBP/p300 HAT maintains the gene network critical for β cell identity and functional maturity.

Authors - Linlin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kamal (Verified Customer) (07-05-2023)

Works with 1X TBST for WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Liver tissue