HNF1A Polyclonal antibody

HNF1A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, co-IP, chIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22426-1-AP

Synonyms

HNF 1 alpha, HNF 1A, HNF1, HNF1 homeobox A, HNF1A, LFB1, MODY3, TCF 1, TCF1, Transcription factor 1

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Validation Data Gallery

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  WB analysis of rat liver using 22426-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22426-1-AP (HNF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 22426-1-AP

  IP Result of anti-HNF1A (IP:22426-1-AP, 4ug; Detection:22426-1-AP 1:800) with mouse liver tissue lysate 3600ug.

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Tested Applications

• Positive WB detected in: rat liver tissue, SMMC-7721 cells
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 22426-1-AP is HNF1A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNF1A fusion protein Ag18188
• Full Name: HNF1 homeobox A
• Calculated molecular weight: 631 aa, 67 kDa
• Observed molecular weight: 67 kDa
• GenBank accession number: BC104910
• Gene symbol: HNF1A
• Gene ID (NCBI): 6927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Publications

Species	Application	Title
human	WB,co-IP	Theranostics; Small Heterodimer Partner Regulates Circadian Cytochromes p450 and Drug-Induced Hepatotoxicity.; Authors - Tianpeng Zhang
human	chIP,WB	FEBS Lett; Hepatocyte nuclear factor 1 alpha (HNF1A) regulates transcription of O-GlcNAc transferase in a negative feedback mechanism.; Authors - Chuanhui Zhang
rat	WB	Acta Pharmacol Sin; Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats.; Authors - Zhen-Li Su
human	WB	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng