HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig, Pig, Rabbit and More (4)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

43

CloneNo.

2D10A5

Reviews (2)

Cat no : 66743-1-Ig

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32

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Validation Data Gallery

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  WB analysis using 66743-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66743-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66743-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66743-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66743-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66743-1-Ig (HO-1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66743-1-Ig (HO-1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver using 66743-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, pig spleen tissue, NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells, pig liver tissue, rabbit liver tissue, rat liver tissue, rat spleen tissue, HeLa cells, A549 cells
• Positive IHC detected in: human liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human liver tissue

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 38 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

66743-1-Ig targets HO-1/HMOX1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Pig, Rabbit
• Cited Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HO-1/HMOX1 fusion protein Ag21296
• Full Name: heme oxygenase (decycling) 1
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC001491
• Gene symbol: HMOX1
• Gene ID (NCBI): 3162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Publications

Species	Application	Title
rat	WB	Biomed Res Int; Effect of Quercetin on PC12 Alzheimer's Disease Cell Model Induced by Aβ25-35 and Its Mechanism Based on Sirtuin1/Nrf2/HO-1 Pathway.; Authors - Xinjun Yu
human	WB	Oxid Med Cell Longev; Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.; Authors - Ning Ma
rat	WB	Acta Pharmacol Sin; CZ-7, a new derivative of Claulansine F, ameliorates 2VO-induced vascular dementia in rats through a Nrf2-mediated antioxidant responses.; Authors - Dan-Dan Liu
human	WB	Acta Pharmacol Sin; Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.; Authors - Jia-Wei Li
mouse	WB,IHC	Oxid Med Cell Longev; Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2; Authors - Jie Jin ; KD Validated
mouse	IF	Cell Death Discov; Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1.; Authors - Shishi Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (08-17-2021)

HO-1 expression was revealed with a donkey anti-mouse 547 red secondary antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: hepG2

HO-1/HMOX1 Antibody Immunofluorescence validation (1/100 dilution) in hepG2 (Cat no:66743-1-Ig)

FH

Marta (Verified Customer) (09-29-2020)

Western blotting of Hmox1 in mouse liver. Used at a 1:1000 dilution in 3% milk and TBST overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

HO-1/HMOX1 Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:66743-1-Ig)