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  • KD/KO Validated

HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig, Pig, Rabbit and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2D10A5

Cat no : 66743-1-Ig

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32



Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, pig spleen tissue, HepG2 cells, HeLa cells, HSC-T6 cells, rat liver tissue, rat spleen tissue, pig liver tissue, rabbit liver tissue
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue, rat liver tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66743-1-Ig targets HO-1/HMOX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Pig, Rabbit
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HO-1/HMOX1 fusion protein Ag21296
Full Name heme oxygenase (decycling) 1
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001491
Gene symbol HMOX1
Gene ID (NCBI) 3162
RRIDAB_2882091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
IHC protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
IF protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
FC protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Adv Healthc Mater

Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing.

Authors - Qiuyue Ding
mouseWB

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
humanWB,IHC,IP

Oncogene

The deubiquitinase USP7 regulates oxidative stress through stabilization of HO-1.

Authors - Ming Gao
humanWB

Cell Biosci

Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth

Authors - Hongting Zhao
ratWB

Oxid Med Cell Longev

Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue

Authors - Guo-Jie Han
humanWB

Oxid Med Cell Longev

Peptides Isolated from Yak Milk Residue Exert Antioxidant Effects through Nrf2 Signal Pathway.

Authors - Feiyan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Does not work. Non-specific band at 70 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Heart Tissue
HO-1/HMOX1 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:66743-1-Ig)
FH

Ayse Tarbin (Verified Customer) (03-16-2023)

It was a gift antibody. It worked perfectly after 2h incubation at room temp. Definately I will consider to buy it.

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:2000 in 5% milk in TBST
  • Cell Tissue Type: Mouse satellite cells
HO-1/HMOX1 Antibody Western Blot,Cell culture validation (1:2000 in 5% milk in TBST dilution) in Mouse satellite cells (Cat no:66743-1-Ig)
FH

Hala (Verified Customer) (08-17-2021)

HO-1 expression was revealed with a donkey anti-mouse 547 red secondary antibody

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: hepG2
HO-1/HMOX1 Antibody Immunofluorescence validation (1/100 dilution) in hepG2 (Cat no:66743-1-Ig)
FH

Marta (Verified Customer) (09-29-2020)

Western blotting of Hmox1 in mouse liver. Used at a 1:1000 dilution in 3% milk and TBST overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver
HO-1/HMOX1 Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:66743-1-Ig)