Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, pig spleen tissue, HepG2 cells, HeLa cells, HSC-T6 cells, rat liver tissue, rat spleen tissue, pig liver tissue, rabbit liver tissue |
| Positive IHC detected in | human liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, rat liver tissue |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 137 publications below |
| IHC | See 12 publications below |
| IF | See 14 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
66743-1-Ig targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig, sheep |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HO-1/HMOX1 fusion protein Ag21296 Predict reactive species |
| Full Name | heme oxygenase (decycling) 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC001491 |
| Gene Symbol | HO-1 |
| Gene ID (NCBI) | 3162 |
| RRID | AB_2882091 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09601 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| IHC protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| IF protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis | ||
Adv Healthc Mater Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing. | ||
Biomaterials MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system. | ||
J Nanobiotechnology Real-world of Limosilactobacillus reuteri in mitigation of acute experimental colitis | ||
Clin Immunol Potential therapeutic effect of dimethyl fumarate on Treg/Th17 cell imbalance in biliary atresia | ||
Oncogene The deubiquitinase USP7 regulates oxidative stress through stabilization of HO-1. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marina (Verified Customer) (07-24-2024) | great antibody. Incubation with primary antibody (1:3000) ON at 4C. Incubation with secondary antibody (mouse) at RT for 2h.
 |
FH Brice-Emmanuel (Verified Customer) (12-18-2023) | Does not work. Non-specific band at 70 kDa
 |
FH Ayse Tarbin (Verified Customer) (03-16-2023) | It was a gift antibody. It worked perfectly after 2h incubation at room temp. Definately I will consider to buy it.
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FH Hala (Verified Customer) (08-17-2021) | HO-1 expression was revealed with a donkey anti-mouse 547 red secondary antibody
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FH Marta (Verified Customer) (09-29-2020) | Western blotting of Hmox1 in mouse liver. Used at a 1:1000 dilution in 3% milk and TBST overnight at 4 degrees.
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