HO-1/HMOX1 Monoclonal antibody
HO-1/HMOX1 Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig and More (4)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
2D10A5
Cat no : 66743-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, pig spleen tissue, NIH/3T3 cells, RAW 264.7 cells, rat spleen tissue, HeLa cells, A549 cells, HepG2 cells, HSC-T6 cells |
| Positive IHC detected in | human liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
| IHC | See 2 publications below |
Product Information
66743-1-Ig targets HO-1/HMOX1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HO-1/HMOX1 fusion protein Ag21296 |
| Full Name | heme oxygenase (decycling) 1 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC001491 |
| Gene symbol | HMOX1 |
| Gene ID (NCBI) | 3162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| IHC protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| IF protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Res Int Effect of Quercetin on PC12 Alzheimer's Disease Cell Model Induced by Aβ25-35 and Its Mechanism Based on Sirtuin1/Nrf2/HO-1 Pathway. | ||
Oxid Med Cell Longev Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK. | ||
Acta Pharmacol Sin CZ-7, a new derivative of Claulansine F, ameliorates 2VO-induced vascular dementia in rats through a Nrf2-mediated antioxidant responses. | ||
Acta Pharmacol Sin Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1. | ||
Med Sci Monit Activation of the Nuclear Erythroid 2-Related Factor 2 Antioxidant Responsive Element (Nrf2-ARE) Signaling Pathway Alleviates Acute Graft-Versus-Host Disease by Reducing Oxidative Stress and Inhibiting Infiltration of Inflammatory Cells in an Allogeneic Stem Cell Transplantation Mouse Model. | ||
Med Sci Monit Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marta (Verified Customer) (09-29-2020) | Western blotting of Hmox1 in mouse liver. Used at a 1:1000 dilution in 3% milk and TBST overnight at 4 degrees.
 |
