HPRT Polyclonal antibody

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  WB analysis using 15059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15059-1-AP

  WB result of HPRT antibody (15059-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT transfected HEK-293 cells.

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  WB analysis of mouse liver using 15059-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15059-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15059-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15059-1-AP

  IP result of anti-HPRT (IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:6000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 15059-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HPRT antibody (15059-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of HeLa using 15059-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HPRT (15059-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

HPRT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15059-1-AP

Publications(34)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRTase, HGPRT, EC:2.4.2.8

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, A549 cells, mouse liver tissue, MCF-7 cells, HepG2 cells, Jurkat cells, Y79 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human pancreas tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 31 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

15059-1-AP targets HPRT in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPRT fusion protein Ag7044
• Full Name: hypoxanthine phosphoribosyltransferase 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC000578
• Gene Symbol: HPRT1
• Gene ID (NCBI): 3251
• RRID: AB_10638622
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00492
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.

Publications

Species	Application	Title
human	WB	J Clin Invest; Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.; Authors - Xiaoyan Yang
rat	WB	J Clin Invest; ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.; Authors - Hidemichi Kouzu
mouse	WB	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
human,mouse	WB	Br J Cancer; Blocking of oestrogen signals improves anti-tumour effect regardless of oestrogen receptor alpha expression in cancer cells; Authors - Nabeel Kajihara
human	WB	EMBO Rep; MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.; Authors - Gongwei Wu
mouse	WB	Elife; Aging is associated with increased brain iron through cortex-derived hepcidin expression.; Authors - Tatsuya Sato