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- KD/KO Validated
HPRT1 Polyclonal antibody
HPRT1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 15059-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse liver tissue, A549 cells, MCF-7 cells, HepG2 cells, Jurkat cells, Y79 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human brain tissue, human pancreas tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 21 publications below |
IF | See 1 publications below |
Product Information
15059-1-AP targets HPRT1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HPRT1 fusion protein Ag7044 |
Full Name | hypoxanthine phosphoribosyltransferase 1 |
Calculated molecular weight | 25 kDa |
Observed molecular weight | 24-28 kDa |
GenBank accession number | BC000578 |
Gene symbol | HPRT1 |
Gene ID (NCBI) | 3251 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HPRT1, also named HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HPRT1 antibody 15059-1-AP | Download protocol |
IHC protocol for HPRT1 antibody 15059-1-AP | Download protocol |
IF protocol for HPRT1 antibody 15059-1-AP | Download protocol |
IP protocol for HPRT1 antibody 15059-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Clin Invest Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2. | ||
J Clin Invest ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice. | ||
EMBO Rep MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation. | ||
Elife Aging is associated with increased brain iron through cortex-derived hepcidin expression. | ||
Neurobiol Dis Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin | ||