HPRT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HPRT1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 15059-1-AP

Synonyms

HGPRT, HGPRTase, HPRT, HPRT1

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Validation Data Gallery

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  WB analysis using 15059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15059-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15059-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15059-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15059-1-AP

  IP Result of anti-HPRT1 (IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 15059-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15059-1-AP (HPRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15059-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HPRT1 (15059-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, A549 cells, MCF-7 cells, HepG2 cells, Jurkat cells, Y79 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human brain tissue, human pancreas tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IF: See 1 publications below

Product Information

15059-1-AP targets HPRT1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPRT1 fusion protein Ag7044
• Full Name: hypoxanthine phosphoribosyltransferase 1
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 24-28 kDa
• GenBank accession number: BC000578
• Gene symbol: HPRT1
• Gene ID (NCBI): 3251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPRT1, also named HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.

Publications

Species	Application	Title
human	WB	J Clin Invest; Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.; Authors - Xiaoyan Yang
rat	WB	J Clin Invest; ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.; Authors - Hidemichi Kouzu
human	WB	EMBO Rep; MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.; Authors - Gongwei Wu
mouse	WB	Elife; Aging is associated with increased brain iron through cortex-derived hepcidin expression.; Authors - Tatsuya Sato
mouse	WB	Neurobiol Dis; Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin; Authors - Daniel Fil
mouse	WB	Sci Rep; Generation of Hprt-disrupted rat through mouse←rat ES chimeras.; Authors - Ayako Isotani ; KO Validated