HPS4 Polyclonal antibody

HPS4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14627-1-AP

Synonyms

bK1048E9.4, bK1048E9.5, Hermansky Pudlak syndrome 4, HPS4, KIAA1667, LE, Light ear protein homolog

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Validation Data Gallery

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  WB analysis using 14627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14627-1-AP (HPS4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14627-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14627-1-AP (HPS4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14627-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14627-1-AP (HPS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Hela cells using 14627-1-AP

  N/A.

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 14627-1-AP

  Immunofluorescent analysis of HepG2 cells, using HPS4 antibody 14627-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, A375 cells, HeLa cells, K-562 cells
• Positive IP detected in: Hela cells, IP result of anti-HPS4 (14267-1-AP for IP and Detection) with HeLa cell lysate.
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

14627-1-AP targets HPS4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPS4 fusion protein Ag6202
• Full Name: Hermansky-Pudlak syndrome 4
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 70-90 kDa
• GenBank Accession Number: BC065030
• Gene Symbol: HPS4
• Gene ID (NCBI): 89781
• RRID: AB_2878071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hermansky-Pudlak syndrome (HPS) is a genetic disease characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. HPS1 and HPS4 are the most frequently mutated genes associated with HPS in humans. Both of HPS1 and HPS4 are components of two complexes involved in biogenesis of melanosome and lysosome-related organelles: BLOC-3 and BLOC-4. HPS4 is supposed to interact with HPS1 and stabilize HPS1. The human HPS4 migrates at about 90 kDa on SDS-PAGE, versus its predicated molecular mass of 77 kDa.

Publications

Species	Application	Title
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook
human	WB	PLoS One; Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.; Authors - Joshi Stephen
mouse	WB	Gene; Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.; Authors - Trieu-Duc Vu
human	WB	Mol Genet Metab; In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.; Authors - Yasuhiro Ikawa