HRD1/SYVN1 Monoclonal antibody

HRD1/SYVN1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

2C8A7

Cat no : 67488-1-Ig

Synonyms

HRD1, HRD1/SYVN1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1

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Validation Data Gallery

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  WB analysis using 67488-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67488-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67488-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67488-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67488-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67488-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRD1/SYVN1 antibody (67488-1-Ig, Clone: 2C8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, 4T1 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Product Information

67488-1-Ig targets HRD1/SYVN1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HRD1/SYVN1 fusion protein Ag4456
• Full Name: synovial apoptosis inhibitor 1, synoviolin
• Calculated Molecular Weight: 617 aa, 68 kDa
• Observed Molecular Weight: 68-76 kDa
• GenBank Accession Number: BC030530
• Gene Symbol: SYVN1
• Gene ID (NCBI): 84447
• RRID: AB_2882714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Publications

Species	Application	Title
mouse	IF	Cell Mol Life Sci; S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.; Authors - Yifei Sun
mouse	WB	FASEB J; UFMylation of HRD1 regulates endoplasmic reticulum homeostasis; Authors - Hui Luo
goat	WB	Int J Mol Sci; Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods; Authors - Zhipeng Sun
mouse	WB,IF	Eur J Pharmacol; Down-regulation of Hrd1 protects against myocardial ischemia-reperfusion injury by regulating PPARα to prevent oxidative stress, endoplasmic reticulum stress, and cellular apoptosis; Authors - Boyu Xia ; KD Validated
human	WB,IF	Clin Transl Med; Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration; Authors - Jiarui Li