HRD1/SYVN1 Monoclonal antibody

HRD1/SYVN1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


Human, Mouse, Rat and More (3)







Cat no : 67488-1-Ig


HRD1, HRD1/SYVN1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1

Tested Applications

Positive WB detected inA549 cells, 4T1 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67488-1-Ig targets HRD1/SYVN1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, goat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HRD1/SYVN1 fusion protein Ag4456
Full Name synovial apoptosis inhibitor 1, synoviolin
Calculated Molecular Weight 617 aa, 68 kDa
Observed Molecular Weight 68-76 kDa
GenBank Accession NumberBC030530
Gene Symbol HRD1
Gene ID (NCBI) 84447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).


Product Specific Protocols
WB protocol for HRD1/SYVN1 antibody 67488-1-IgDownload protocol
IHC protocol for HRD1/SYVN1 antibody 67488-1-IgDownload protocol
IF protocol for HRD1/SYVN1 antibody 67488-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Mol Life Sci

S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

Authors - Yifei Sun


UFMylation of HRD1 regulates endoplasmic reticulum homeostasis

Authors - Hui Luo

Int J Mol Sci

Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods

Authors - Zhipeng Sun

Eur J Pharmacol

Down-regulation of Hrd1 protects against myocardial ischemia-reperfusion injury by regulating PPARα to prevent oxidative stress, endoplasmic reticulum stress, and cellular apoptosis

Authors - Boyu Xia
  • KD Validated

Clin Transl Med

Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration

Authors - Jiarui Li