Tested Applications
| Positive WB detected in | A549 cells, 4T1 cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, rat liver tissue, mouse brain tissue, mouse liver tissue, rat lung tissue, human ovary cancer tissue, rat brain tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67488-1-Ig targets HRD1/SYVN1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4456 Product name: Recombinant human HRD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 245-617 aa of BC030530 Sequence: IRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPPPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGAATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH Predict reactive species |
| Full Name | synovial apoptosis inhibitor 1, synoviolin |
| Calculated Molecular Weight | 617 aa, 68 kDa |
| Observed Molecular Weight | 68-76 kDa |
| GenBank Accession Number | BC030530 |
| Gene Symbol | HRD1 |
| Gene ID (NCBI) | 84447 |
| RRID | AB_2882714 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86TM6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HRD1/SYVN1 antibody 67488-1-Ig | Download protocol |
| IHC protocol for HRD1/SYVN1 antibody 67488-1-Ig | Download protocol |
| WB protocol for HRD1/SYVN1 antibody 67488-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HRD1/SYVN1 Monoclonal antibody (2C8A7), catalog number 67488-1-Ig, has 10 citations. These appear in journals including Nature Cardiovascular Research, Clinical & Translational Medicine, Cell Proliferation, Cell & Molecular Life Sciences, European Journal of Pharmacology, International Journal of Molecular Sciences, European Journal of Nutrition, FASEB Journal, Clinical and Experimental Pharmacology and Physiology, and Heliyon. Research fields span cardiovascular disease, ferroptosis, kidney injury, myocardial ischemia-reperfusion, osteoporosis, endoplasmic reticulum homeostasis, and pulmonary fibrosis.
| Species | Application | Title |
|---|---|---|
Nat Cardiovasc Res p22(phox) prevents the oxidation of SERCA2a and stabilizes it in the heart. | ||
Clin Transl Med Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration | ||
Cell Prolif The Syvn1 inhibits neuronal cell ferroptosis by activating Stat3/Gpx4 axis in rat with spinal cord injury.
| ||
Cell Mol Life Sci S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α. | ||
Eur J Pharmacol Down-regulation of Hrd1 protects against myocardial ischemia-reperfusion injury by regulating PPARα to prevent oxidative stress, endoplasmic reticulum stress, and cellular apoptosis
| ||
Int J Mol Sci Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods |
Reviews
What customers say
The antibody received a 4-star rating for Western Blot applications. The reviewer confirmed that the HRD1/SYVN1 Mouse Monoclonal Antibody performed as expected, producing reliable results in their WB experiments. Overall, the feedback indicates the product meets standard expectations for its intended use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
ira (Verified Customer) (10-22-2026) | HRD1/SYVN1 Mouse McAb is working as expected for western blots
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