HRD1/SYVN1 Monoclonal antibody

HRD1/SYVN1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2C8A7

Cat no : 67488-1-Ig

Synonyms

HRD1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1



Tested Applications

Positive WB detected inA549 cells, Jurkat cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67488-1-Ig targets HRD1/SYVN1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HRD1/SYVN1 fusion protein Ag4456
Full Name synovial apoptosis inhibitor 1, synoviolin
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68-76 kDa
GenBank accession numberBC030530
Gene symbol SYVN1
Gene ID (NCBI) 84447
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Protocols

Product Specific Protocols
WB protocol for HRD1/SYVN1 antibody 67488-1-IgDownload protocol
IHC protocol for HRD1/SYVN1 antibody 67488-1-IgDownload protocol
IF protocol for HRD1/SYVN1 antibody 67488-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols