CoraLite®594-conjugated HRD1/SYVN1 Monoclonal antibody

HRD1/SYVN1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C8A7

Cat no : CL594-67488

Synonyms

HRD1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67488-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67488 targets HRD1/SYVN1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HRD1/SYVN1 fusion protein Ag4456
Full Name synovial apoptosis inhibitor 1, synoviolin
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68-76 kDa
GenBank accession numberBC030530
Gene symbol SYVN1
Gene ID (NCBI) 84447
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Protocols

Product Specific Protocols
IF protocol for CL594 HRD1/SYVN1 antibody CL594-67488Download protocol
Standard Protocols
Click here to view our Standard Protocols