HRPT2, CDC73 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HRPT2, CDC73 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4B4H2

Cat no : 66490-1-PBS

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin

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Validation Data Gallery

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  WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

  WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66490-1-Ig (same clone as 66490-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66490-1-Ig (same clone as 66490-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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Product Information

66490-1-PBS targets HRPT2, CDC73 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HRPT2, CDC73 fusion protein Ag2959
• Full Name: cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 531 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014351
• Gene Symbol: HRPT2/CDC73
• Gene ID (NCBI): 79577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).