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HRPT2, CDC73 Monoclonal antibody

HRPT2, CDC73 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, Pig, Mouse, Rat and More (1)







Cat no : 66490-1-Ig


C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin

Tested Applications

Positive WB detected inpig brain tissue, LNCaP cells, HeLa cells, mouse brain tissue, HEK-293 cells, Caco-2 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66490-1-Ig targets HRPT2, CDC73 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HRPT2, CDC73 fusion protein Ag2959
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated Molecular Weight 531 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014351
Gene Symbol CDC73
Gene ID (NCBI) 79577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).


Product Specific Protocols
WB protocol for HRPT2, CDC73 antibody 66490-1-IgDownload protocol
IHC protocol for HRPT2, CDC73 antibody 66490-1-IgDownload protocol
IF protocol for HRPT2, CDC73 antibody 66490-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao

Horm Res Paediatr

A Familial Case of a Whole Germline CDC73 Deletion Discordant for Primary Hyperparathyroidism.

Authors - Naomi Hatabu