HRPT2, CDC73 Monoclonal antibody

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• KD/KO Validated

HRPT2, CDC73 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

4B4H2

Cat no : 66490-1-Ig

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin

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Validation Data Gallery

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  WB analysis of HeLa using 66490-1-Ig

  WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells.

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  WB analysis using 66490-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis using 66490-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 66490-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66490-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66490-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66490-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66490-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 66490-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, LNCaP cells, HeLa cells, mouse brain tissue, HEK-293 cells, Caco-2 cells, RAW 264.7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

66490-1-Ig targets HRPT2, CDC73 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HRPT2, CDC73 fusion protein Ag2959
• Full Name: cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 531 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014351
• Gene Symbol: HRPT2/CDC73
• Gene ID (NCBI): 79577
• RRID: AB_2881855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

Publications

Species	Application	Title
mouse	WB	J Proteomics; To explore the regulatory role of Wnt/P53/Caspase3 signal in mouse ovarian development based on LFQ proteomics; Authors - Yabo Ma
mouse	WB	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao
human		bioRxiv; Resistance of estrogen receptor function to BET bromodomain inhibition is mediated by transcriptional coactivator cooperativity; Authors - Sicong Zhang
	IHC	Horm Res Paediatr; A Familial Case of a Whole Germline CDC73 Deletion Discordant for Primary Hyperparathyroidism.; Authors - Naomi Hatabu