HSP90 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSP90 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

214

Reviews (4)

Cat no : 13171-1-AP

Synonyms

Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

---

Validation Data Gallery

• 

  WB analysis using 13171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 13171-1-AP

  IP Result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with K-562 cells lysate 2000ug.

• 

  IP experiment of HeLa using 13171-1-AP

  IP Result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with HeLa cells lysate 4400ug.

• 

  IHC staining of human liver cancer using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13171-1-AP (HSP90 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13171-1-AP (HSP90 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 13171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13171-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 13171-1-AP

  1X10^6 HeLa cells were stained with 0.20ug HSP90 antibody (13171-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

• 

  FC experiment of A549 using 13171-1-AP

  1X10^6 A549 cells were stained with 0.20ug HSP90 antibody (13171-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: K-562 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 191 publications below
• IHC: See 9 publications below
• IF: See 14 publications below
• IP: See 12 publications below
• ELISA: See 3 publications below
• CoIP: See 11 publications below
• RIP: See 2 publications below

Product Information

13171-1-AP targets HSP90 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, carp, fish, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSP90 fusion protein Ag3826
• Full Name: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Calculated molecular weight: 853 aa, 90 kDa
• Observed molecular weight: 90 kDa
• GenBank accession number: BC023006
• Gene symbol: HSP90AA1
• Gene ID (NCBI): 3320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Publications

Species	Application	Title
human	WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long
	CoIP	Nat Cell Biol; LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.; Authors - Gaopeng Li
		Mol Cell; SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.; Authors - Tianshi Wang
mouse	WB	Neuron; UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.; Authors - Kejing Zhang
human	WB	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
human	WB	Nat Commun; FUNDC2 promotes liver tumorigenesis by inhibiting MFN1-mediated mitochondrial fusion.; Authors - Shuaifeng Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Satyendra (Verified Customer) (07-06-2022)

Works very nicely with CEF, DF-1 and VeroE6 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: VeroE6 cells

FH

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: ARPE-19

HSP90 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:13171-1-AP)

FH

Ekaterini (Verified Customer) (02-16-2022)

The best antibody to use for loading control. Always clear and it is not affected by drug treatments.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ALL cell lines

FH

Xiaoping (Verified Customer) (10-22-2018)

This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human neuron and mouse fibroblast samples

HSP90 Antibody Western Blot, validation (1:1000 dilution) in Human neuron and mouse fibroblast samples (Cat no:13171-1-AP)

Related Products

HSP70 Polyclonal antibody

10995-1-AP

HSP70 Monoclonal antibody

66183-1-Ig

Alpha Synuclein Polyclonal antibody

10842-1-AP

HSP60 Polyclonal antibody

15282-1-AP

HSP60 Monoclonal antibody

66041-1-Ig