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HSP90 Polyclonal antibody
HSP90 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 13171-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||K-562 cells, HeLa cells|
|Positive IHC detected in||human liver cancer tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells, A549 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13171-1-AP targets HSP90 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, carp, fish, pig, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSP90 fusion protein Ag3826|
|Full Name||heat shock protein 90kDa alpha (cytosolic), class A member 1|
|Calculated molecular weight||853 aa, 90 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC023006|
|Gene ID (NCBI)||3320|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.
|Product Specific Protocols|
|WB protocol for HSP90 antibody 13171-1-AP||Download protocol|
|IHC protocol for HSP90 antibody 13171-1-AP||Download protocol|
|IF protocol for HSP90 antibody 13171-1-AP||Download protocol|
|IP protocol for HSP90 antibody 13171-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Satyendra (Verified Customer) (07-06-2022)
Works very nicely with CEF, DF-1 and VeroE6 cells
Guorong (Verified Customer) (03-22-2022)
Excellent performance with a specific band of expected size
Ekaterini (Verified Customer) (02-16-2022)
The best antibody to use for loading control. Always clear and it is not affected by drug treatments.
Xiaoping (Verified Customer) (10-22-2018)
This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.