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HSP90 Polyclonal antibody
HSP90 Polyclonal Antibody for WB, IP, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IP, IF, FC, RIP, IHC, CoIP, ELISA
Conjugate
Unconjugated
259
Cat no : 13171-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue |
Positive IP detected in | K-562 cells, HeLa cells |
Positive IHC detected in | human liver cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells, A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) | FC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
13171-1-AP targets HSP90 in WB, IP, IF, FC, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, carp, fish, pig, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HSP90 fusion protein Ag3826 |
Full Name | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
Calculated Molecular Weight | 853 aa, 90 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC023006 |
Gene Symbol | HSP90AA1 |
Gene ID (NCBI) | 3320 |
RRID | AB_2120924 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HSP90 antibody 13171-1-AP | Download protocol |
IHC protocol for HSP90 antibody 13171-1-AP | Download protocol |
IF protocol for HSP90 antibody 13171-1-AP | Download protocol |
IP protocol for HSP90 antibody 13171-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment | ||
Nat Commun The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells | ||
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology | ||
Mol Cell SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Satyendra (Verified Customer) (07-06-2022) | Works very nicely with CEF, DF-1 and VeroE6 cells
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FH Guorong (Verified Customer) (03-22-2022) | Excellent performance with a specific band of expected size
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FH Ekaterini (Verified Customer) (02-16-2022) | The best antibody to use for loading control. Always clear and it is not affected by drug treatments.
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FH Xiaoping (Verified Customer) (10-22-2018) | This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.
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