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HSP90 Polyclonal antibody

HSP90 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (5)





Cat no : 13171-1-AP


Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inK-562 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells, A549 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13171-1-AP targets HSP90 in WB,IP,IHC,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, carp, fish, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP90 fusion protein Ag3826
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated Molecular Weight 853 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC023006
Gene Symbol HSP90AA1
Gene ID (NCBI) 3320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.


Product Specific Protocols
WB protocol for HSP90 antibody 13171-1-APDownload protocol
IHC protocol for HSP90 antibody 13171-1-APDownload protocol
IF protocol for HSP90 antibody 13171-1-APDownload protocol
IP protocol for HSP90 antibody 13171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long

Nat Cell Biol

LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.

Authors - Gaopeng Li

Nat Commun

FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment

Authors - Zongchao Sun

Nat Commun

The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells

Authors - Xiaofeng Liao

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar

Mol Cell

SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.

Authors - Tianshi Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Satyendra (Verified Customer) (07-06-2022)

Works very nicely with CEF, DF-1 and VeroE6 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: VeroE6 cells

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: ARPE-19
HSP90 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:13171-1-AP)

Ekaterini (Verified Customer) (02-16-2022)

The best antibody to use for loading control. Always clear and it is not affected by drug treatments.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ALL cell lines

Xiaoping (Verified Customer) (10-22-2018)

This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human neuron and mouse fibroblast samples
HSP90 Antibody Western Blot, validation (1:1000 dilution) in Human neuron and mouse fibroblast samples (Cat no:13171-1-AP)