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GRP94 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10979-1-AP

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Con: 34 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

GRP94 Antibody WB HEK-293 cells 10979-1-AP

WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells.

GRP94 Antibody IHC human breast cancer tissue 10979-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 10979-1-AP(HSP90B1 antibody) at dilution of 1:50 (under 10x lens)

GRP94 Antibody IHC human breast cancer tissue 10979-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 10979-1-AP(HSP90B1 antibody) at dilution of 1:50 (under 40x lens)

GRP94 Antibody IP MCF-7 cells 10979-1-AP

IP Result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.

GRP94 Antibody IF MCF-7 cells 10979-1-AP

Immunofluorescent analysis of MCF-7 cells using 10979-1-AP(GRP94 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

GRP94 Antibody FC MCF-7 cells 10979-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug GRP94 antibody (10979-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, A549 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells, HEK-293 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GRP94 fusion protein Ag1439
Full name:
heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight:
96 kDa
Observed molecular weight:
94 kDa
GenBank accession number:
BC009195
Gene ID (NCBI):
7184
Gene symbol
HSP90B1
Synonyms
ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

Background

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.


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