GRP94 Antibody

• Featured Product
• KD/KO Validated

GRP94 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10979-1-AP

Synonyms

ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

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Validation Data Gallery

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  WB analysis of HEK-293 cells using 10979-1-AP

  WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells.

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  IHC staining of human breast cancer tissue using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP(HSP90B1 antibody) at dilution of 1:50 (under 10x lens)

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  IHC staining of human breast cancer tissue using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP(HSP90B1 antibody) at dilution of 1:50 (under 40x lens)

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  IP experiment of MCF-7 cells using 10979-1-AP

  IP Result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.

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  IF staining of MCF-7 cells using 10979-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10979-1-AP(GRP94 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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  FC experiment of MCF-7 cells using 10979-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug GRP94 antibody (10979-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; ; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, HEK-293 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

10979-1-AP targets GRP94 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Reactivity: human, mouse, rat
• Cited Species: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRP94 fusion protein Ag1439
• Full Name: heat shock protein 90kDa beta (Grp94), member 1
• Calculated molecular weight: 96 kDa
• Observed molecular weight: 94 kDa
• GenBank accession number: BC009195
• Gene symbol: HSP90B1
• Gene ID (NCBI): 7184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Publications

Species	Application	Title
rat	WB	Mol Med Rep; Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.; Authors - Maojun Liu