GRP94 Polyclonal antibody

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• GRP94 Polyclonal antibody (10979-1-AP)

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• KD/KO Validated

GRP94 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 10979-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

HSP90B1, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, GP96

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  WB analysis using 10979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10979-1-AP (GRP94 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 10979-1-AP

  WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells..

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 10979-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 10979-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 10979-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 10979-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 10979-1-AP

  IP result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human breast cancer using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human appendicitis using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse pancreas using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 10979-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug GRP94 Polyclonal antibody (10979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue, human appendicitis tissue
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10979-1-AP targets GRP94 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1439

  Product name: Recombinant human GRP94 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC009195

  Sequence: MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAARRR

• Full Name: heat shock protein 90kDa beta (Grp94), member 1
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC009195
• Gene Symbol: GRP94
• Gene ID (NCBI): 7184
• RRID: AB_2119057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14625
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Publications

What published studies show

The GRP94 Polyclonal antibody (Cat# 10979-1-AP) has 8 citations published in journals including Nature Communications, Journal of Neuroinflammation, Molecular Medicine Reports, Carcinogenesis, Journal of Clinical Medicine, Journal of Immunology Research, Veterinary Microbiology, and Experimental Brain Research. The associated research fields span ER stress and neurodegeneration (Parkinson's disease, Pelizaeus-Merzbacher disease), cardiac fibrosis in diabetes, cancer biomarker discovery, B-cell lymphoma prognosis, pulmonary fibrosis, viral replication, and neuronal injury protection.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Commun; Integrated stress response inhibition prolongs the lifespan of a Pelizaeus-Merzbacher disease mouse model by increasing oligodendrocyte survival.; Authors - Yanan Chen
mouse	WB	J Neuroinflammation; TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.; Authors - Na Liu
rat	WB	Mol Med Rep; Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.; Authors - Maojun Liu
human	WB	Carcinogenesis; Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis; Authors - Huajie Zong
human	WB	J Clin Med; Possible New Histological Prognostic Index for Large B-Cell Lymphoma; Authors - Hideaki Nitta
mouse	WB,IHC,IF	J Immunol Res; Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis; Authors - Qiaohui Hong